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The Journal of Cell Biology, Vol 53, 654-661, Copyright © 1972 by Rockefeller University Press

ARTICLE

PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS

Fritz A. Henn 1, Hans-Arne Hansson 1, and Anders Hamberger 1

1 From the Institute of Neurobiology, Medical Faculty, University of Göteborg, Göteborg, Sweden.

Dr. Henn's present address is the Department of Psychiatry, Washington University School of Medicine, St. Louis, Missouri 63110.

A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4–5-fold enrichment in plasma membrane markers, and a 10–15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen

Submitted on October 18, 1971
Revised on January 24, 1972


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