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The Journal of Cell Biology, Vol 54, 1-7, Copyright © 1972 by Rockefeller University Press

ARTICLE

RNA METABOLISM IN HELA CELLS AT REDUCED TEMPERATURE : II. Steps in the Processing of Transfer RNA



Ronald H. Stevens 1 and Harold Amos 1

1 From the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Dr. Stevens's present address is the National Institute for Medical Research, London, England.

Incubation of HeLa cells at 24°C results in the modification of the processing of pre-tRNA to tRNA. Both methylation and size reduction were shown to take place in vitro when purified pre-tRNA was subjected to processing in a cytoplasmic extract of HeLa cells The migration of pre-tRNA from the nucleus to the cytoplasm was not significantly altered at 24°C

Submitted on September 27, 1971
Revised on March 27, 1972


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