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The Journal of Cell Biology, Vol 54, 540-555, Copyright © 1972 by Rockefeller University Press

ARTICLE

CHLAMYDOMONAS FLAGELLA : II. The Distribution of Tubulins 1 and 2 in the Outer Doublet Microtubules



G. B. Witman 1, K. Carlson 1, and Joel L. Rosenbaum 1

1 From the Department of Biology, Yale University, New Haven, Connecticut 06520.

Dr. Witman's present address is Whitman Laboratory, Department of Biology, University of Chicago, Chicago, Illinois 60601.

Quantitative ultrastructural analysis and quantitative gel electrophoresis of preparations of selectively solubilized Chlamydomonas outer doublets indicated that tubulins 1 and 2 were present in both the A tubule and the B tubule, and that only tubulin 1 was present in the three protofilaments which form the wall ("partition") between the lumens of the A and B tubules. The data suggested that the remaining protofilaments of the outer doublet were grouped together in pairs containing the same type of tubulin, pairs containing tubulin 1 alternating with pairs containing tubulin 2. These findings were used to construct models for the arrangement of the two tubulins in the outer doublet. Further analysis by isoelectric focusing resolved tubulins 1 and 2 into at least five bands.

Submitted on March 6, 1972
Revised on May 5, 1972


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