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The Journal of Cell Biology, Vol 54, 580-591, Copyright © 1972 by Rockefeller University Press

ARTICLE

CHROMOSOMAL LOCALIZATION OF REPETITIVE DNA IN THE NEWT, TRITURUS

Giuseppina Barsacchi 1 and Joseph G. Gall 1

1 From the Department of Biology, Yale University, New Haven, Connecticut 06520.

Dr Barsacchi's present address is the Istituto di Zoologia e Anatomia Comparata, Università di Pisa, 56100 Pisa, Italy.

The repetitive DNA sequences of the newt, Triturus viridescens, have been studied by nucleic acid hybridization procedures. Complementary RNA was synthesized enzymatically from unfractionated newt DNA. This RNA hybridized strongly to the centromeric regions of both somatic and lampbrush chromosomes It also bound to other loci scattered along the lengths of the chromosomes The amplified ribosomal DNA in the multiple oocyte nucleoli was demonstrated by in situ hybridization

Submitted on February 28, 1972
Revised on May 19, 1972


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