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The Journal of Cell Biology, Vol 54, 598-608, Copyright © 1972 by Rockefeller University Press

ARTICLE

ELECTRON MICROSCOPE STUDIES OF SPERMATOZOA OF RHYNCHOSCIARA SP : I. Disruption of Microtubules by Various Treatments



Jerry W. Shay 1

1 From the Department of Physiology and Cell Biology, The University of Kansas, Lawrence, Kansas 66044.

Dr. Shay's present address is the Department of Molecular, Cellular, and Developmental Biology, The University of Colorado, Boulder, Colorado 80302.

The flagellar complex of the unusual motile spermatozoon of the fungus gnat, Rhynchosciara sp, does not conform to the usual "9 + 2" filament pattern but rather consists of over 350 pairs of filaments (doublet microtubules) distributed in a spiral array. Experiments were designed to disrupt and extract flagellar microtubular components from spermatozoa of the fungus gnat. Pepsin, chymotrypsin, potassium iodide, urea, and heat were used to extract specific portions of microtubule walls Such experiments provide information on the composition of the wall and the existence of wall sites selectively sensitive to various treatments Results obtained include: (a) doublet microtubules are comprised at least in part of protein, and all subunits are probably not identical; (b) a portion of the B subfiber is apparently more sensitive to disruption than other portions of the doublet microtubule; and (c) the ac cessory singlet microtubules may be chemically different from the doublet microtubules

Submitted on March 9, 1972
Revised on May 19, 1972


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