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The Journal of Cell Biology, Vol 55, 104-125, Copyright © 1972 by Rockefeller University Press

ARTICLE

FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes



Ariane Monneron 1, Günter Blobel 1, and George E. Palade 1

1 From The Rockefeller University, New York 10021

0.3–0.5 M MgCl2 was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.

Submitted on February 7, 1972
Revised on June 5, 1972


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