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CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells

Thoru Pederson ¹ and Elliott Robbins ¹

¹ From the Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.

Dr. Pederson's present address is The Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

Measurements of actinomycin-³H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G₂ and mitosis, and then increases gradually throughout the subsequent G₁ interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle.

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This article has been cited by other articles:

• Beall, P., Hazlewood, C., Rao, P. (1976). Nuclear magnetic resonance patterns of intracellular water as a function of HeLa cell cycle. Science 192: 904-907 [Abstract]  

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