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The Journal of Cell Biology, Vol 55, 355-367, Copyright © 1972 by Rockefeller University Press

ARTICLE

COLCEMID INHIBITION OF CELL GROWTH AND THE CHARACTERIZATION OF A COLCEMID-BINDING ACTIVITY IN SACCHAROMYCES CEREVISIAE

James E. Haber 1, John G. Peloquin 1, Harlyn O. Halvorson 1, and Gary G. Borisy 1

1 From the Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706.

Drs. Haber and Halvorson's present address is Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02154.

Under restricted culture conditions, the growth and division of Saccharomyces cerevisiae was inhibited by the antimitotic drug Colcemid; in contrast, the related drug colchicine had no effect. The difference in the sensitivity of yeast to these two agents was not dependent on their ability to permeate the cell but rather reflected an inherent difference in the affinity of the two drugs for a cellular-binding site. The binding moiety was characterized by gel filtration as a macromolecule of approximately 110,000 mol wt with an affinity constant for Colcemid of 0.5 x 104 liters per mole; in addition, this macromolecule was retained by diethylaminoethyl (DEAE) ion exchangers. On the basis of these properties, the Colcemid-binding substance in S. cerevisiae cells was provisionally identified as microtubule subunits.

Submitted on April 28, 1972
Revised on May 25, 1972


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