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The Journal of Cell Biology, Vol 55, 681-695, Copyright © 1972 by Rockefeller University Press

ARTICLE

M BAND PROTEIN : Two Components Isolated from Chicken Breast Muscle



Barbra L. Eaton 1 and Frank A. Pepe 1

1 From the Biochemistry Graduate Group and the Department of Anatomy, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104

M band protein can be specifically extracted from fresh chicken breast muscle myofibrils suspended in 5 mM Tris-HCl pH 8.0. During discontinuous polyacrylamide gel electrophoresis the isolated protein separates into three bands which can be identified as two separate components (A, B) and a complex of the two. When partially purified fractions of the separated components are combined, an increase in the intensity of the band containing the complex can be shown. The polypeptide chain weights of the two components are 100,000 (A) and 40,000 (B) daltons as estimated by sodium dodecyl sulfate- (SDS-) polyacrylamide gel electrophoresis. Antibody prepared against total M band protein stains only the M band of the myofibril and is completely absorbed by M band protein. M band protein also absorbs the M band staining specifically from antibody which stains both I and M bands. Immunodiffusion data indicate that anti-M band is a mixture of two specific antibodies, one against each component.

Submitted on June 7, 1972
Revised on August 2, 1972


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