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The Journal of Cell Biology, Vol 56, 191-205, Copyright © 1973 by Rockefeller University Press

ARTICLE

AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES

M. R. Adelman 1, Gunter Blobel 1, and David D. Sabatini 1

1 From The Rockefeller University, New York 10021

Dr Adelman's present address is the Department of Anatomy, Duke University Medical Center, Durham, North Carolina 27710.

A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.

Submitted on July 10, 1972
Revised on August 16, 1972


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