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The Journal of Cell Biology, Vol 56, 429-433, Copyright © 1973 by Rockefeller University Press

ARTICLE

THE PERSISTENCE OF HEMOPOIETIC STEM CELLS IN VITRO

Russell Meints 1 and Eugene Goldwasser 1

1 From the Argonne Cancer Research Hospital and Department of Biochemistry, University of Chicago, Chicago, Illinois 60637.

Dr. Meints's present address is the Department of Zoology, University of Nebraska, Lincoln, Nebraska 68508.

Cells capable of forming colonies in spleens of irradiated mice (CFU) are lost temporarily when bone marrow cells from rats or mice are maintained in culture. Rat marrow CFU go through a minimum at about 3 days after which there is a slow increase in the number of CFU in culture, reaching a maximum at 9 days. Mouse marrow CFU reach a minimum at 3 days and a maximum at 7 days. Some rat marrow CFU persist in culture for as long as 28 days.

Submitted on June 28, 1972
Revised on September 25, 1972


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