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The Journal of Cell Biology, Vol 56, 434-440, Copyright © 1973 by Rockefeller University Press

ARTICLE

SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation



Irving Finger 1, Patricia Lavanchy 1, and Ann Meany 1

1 From the Department of Biology, Haverford College, Haverford, Pennsylvania 19041

The synthesis of a surface protein has been studied in Paramecium through double-labeling experiments using [14C]- and [3H]leucine-labeled bacteria as the source of radioactive amino acid. Over a 4–5 h incubation period, the turnover rate was found to be higher than that of overall cell protein. In addition, the initial label is apparently utilized during the chase period, being incorporated into protein via a puromycin insensitive pathway.

Submitted on March 24, 1972
Revised on October 9, 1972


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