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The Journal of Cell Biology, Vol 56, 787-796, Copyright © 1973 by Rockefeller University Press

ARTICLE

A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure



P. M. Quinton 1 and C. W. Philpott 1

1 From the Department of Biology, Rice University, Houston, Texas 77006.

Dr. Quinton's present address is the Department of Physiology, University of California at Los Angeles Medical School, Los Angeles, California 90024.

The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37°C in Ringer's solutions containing from 0.1 to 100.0 µg/ml of cationic polymers, and subsequently was fixed with 1% OsO4 and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures.

Submitted on July 28, 1972
Revised on September 28, 1972


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