Spindle and kinetochore morphology of Dictyostelium discoideum

doi:10.1083/jcb.68.1.113

This Article

	Full Text (PDF, 4053K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Moens, P. B.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Moens, P. B.


Social Bookmarking

	What's this?

ARTICLES

Spindle and kinetochore morphology of Dictyostelium discoideum

PB Moens

The metaphase spindle of haploid Dictyostelium discoideum (n = 7) is 2 mum long. It consists of some 20 microtubules which seem continuous between the spindle pole bodies and there are about 20 chromosomal microtubules at each end of the spindle. During anaphase the central spindle elongates and the chromosomal microtubules shorten. The spindle length and structure at this stage suggests that lengthening is caused by elongation as well as parallel sliding of the nonchromosomal microtubules. The nuclear envelope remains mostly intact during mitosis, and nuclear separation through medial constriction takes place when the spindle is 6 mum long. Cytokinesis occurs when the spindle is 10 mum long. At that time the kinetochores double in size. During interphase, the spindle pole body separates from the nucleus to a distance of 0.7 mum, and it returns at the onset of the next prophase when it becomes functionally double, thereby starting the formation of a central spindle. When comparing mitosis in the cellular slime molds Polysphondylium violaceum and D. discoideum, several similarities and some differences are apparent.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Li, H., Chen, Q., Kaller, M., Nellen, W., Graf, R., De Lozanne, A. (2008). Dictyostelium Aurora Kinase Has Properties of both Aurora A and Aurora B Kinases. Eukaryot Cell 7: 894-905 [Abstract] [Full Text]
Muramoto, T., Chubb, J. R. (2008). Live imaging of the Dictyostelium cell cycle reveals widespread S phase during development, a G2 bias in spore differentiation and a premitotic checkpoint. Development 135: 1647-1657 [Abstract] [Full Text]
Kaller, M., Euteneuer, U., Nellen, W. (2006). Differential Effects of Heterochromatin Protein 1 Isoforms on Mitotic Chromosome Distribution and Growth in Dictyostelium discoideum. Eukaryot Cell 5: 530-543 [Abstract] [Full Text]
Graf, R., Euteneuer, U., Ho, T.-H., Rehberg, M. (2003). Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number. Mol. Biol. Cell 14: 4067-4074 [Abstract] [Full Text]
Swanson, A. R., Spiegel, F. W., Cavender, J. C. (2002). Taxonomy, slime molds, and the questions we ask. Mycologia 94: 968-979 [Abstract] [Full Text]
Graf, R. (2002). DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers. J. Cell Sci. 115: 1919-1929 [Abstract] [Full Text]
Yang, M., Ma, H. (2001). Male Meiotic Spindle Lengths in Normal and Mutant Arabidopsis Cells. Plant Physiol. 126: 622-630 [Abstract] [Full Text]
Graf, R, Daunderer, C, Schliwa, M (2000). Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication. J. Cell Sci. 113: 1747-1758 [Abstract]
Ma, S., Trivinos-Lagos, L., Graf, R., Chisholm, R. L. (1999). Dynein Intermediate Chain Mediated Dynein-Dynactin Interaction Is Required for Interphase Microtubule Organization and Centrosome Replication and Separation in Dictyostelium. JCB 147: 1261-1274 [Abstract] [Full Text]
Ueda, M., Schliwa, M., Euteneuer, U. (1999). Unusual Centrosome Cycle in Dictyostelium: Correlation of Dynamic Behavior and Structural Changes. Mol. Biol. Cell 10: 151-160 [Abstract] [Full Text]
Zang, J.-H., Spudich, J. A. (1998). Myosin II localization during cytokinesis occurs by a mechanism that does not require its motor domain. Proc. Natl. Acad. Sci. USA 95: 13652-13657 [Abstract] [Full Text]
Neujahr, R, Albrecht, R, Kohler, J, Matzner, M, Schwartz, J., Westphal, M, Gerisch, G (1998). Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells. J. Cell Sci. 111: 1227-1240 [Abstract]
Euteneuer, U, Graf, R, Kube-Granderath, E, Schliwa, M (1998). Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization. J. Cell Sci. 111: 405-412 [Abstract]
Sabry, J. H., Moores, S. L., Ryan, S., Zang, J.-H., Spudich, J. A. (1997). Myosin Heavy Chain Phosphorylation Sites Regulate Myosin Localization during Cytokinesis in Live Cells. Mol. Biol. Cell 8: 2605-2615 [Abstract] [Full Text]
Zang, J.-H., Cavet, G., Sabry, J. H., Wagner, P., Moores, S. L., Spudich, J. A. (1997). On the Role of Myosin-II in Cytokinesis: Division of Dictyostelium Cells under Adhesive and Nonadhesive Conditions. Mol. Biol. Cell 8: 2617-2629 [Abstract] [Full Text]
Trivinos-Lagos, L, Ohmachi, T, Albrightson, C, Burns, R., Ennis, H., Chisholm, R. (1993). The highly divergent alpha- and beta-tubulins from Dictyostelium discoideum are encoded by single genes. J. Cell Sci. 105: 903-911 [Abstract]