The Journal of Cell Biology, Vol 68, 90-100, Copyright © 1976 by The Rockefeller University Press
Intercellular adhesive selectivity. II. Properties of embryonic chick liver cell-cell adhesion
EJ McGuire
Studies directed at understanding the molecular basis of liver cell
homotypic adhesion are presented. An assay which measures the rate of
adhesion of isotopically labeled (32PO4) embryonic chick liver cells to
liver cell aggregates, described in a companion paper, has been used to
investigate the problem of intercellular adhesive selectivity. Cation
requirements, the effects of various inhibitors of metabolism and protein
synthesis, of chelators (EDTA and EGTA), and the effects of temperature on
liver cell adhesion are reported. Two mechanisms of inhibition of liver
intercellular adhesion are suggested. One involves destruction of
cell-surface adhesion receptors (sensitivity to proteases); the other is an
energy-dependent step which may involve alterations in plasma membrane
conformation and/or membrane fluidity. Finally, a model is suggested for
liver cell-cell adhesion that incorporates the early tissue selectivity of
intercellular adhesion previously reported, followed by a multistep process
which leads to histogenic aggregation.
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