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The Journal of Cell Biology, Vol 68, 389-395, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Glycine-specific synapses in rat spinal cord. Identification by electron microscope autoradiography

DL Price, A Stocks, JW Griffin, A Young and K Peck

Glycine, an inhibitory transmitter in spinal cord, is taken up into specific nerve terminals by means of a unique high-affinity uptake system. In this study, [3H]glycine was directly microinjected into rat ventral horn in vivo and electron microscope autoradiography used to localize the label in various anatomic compartments. Quantiative analysis showed that [3H]glycine labeled a high proportion of axosomatic and axodendritic synapses which presumably act to inhibit spinal motor neurons.
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