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Fluorescence In Vivo Endomicroscopy
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The Journal of Cell Biology, Vol 68, 781-787, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes

S Hoffstein, R Soberman, I Goldstein and G Weissmann

Human neutrophils stimulated by concanavalin A (Con A, 100 microng/ml) contained markedly enhanced numbers of microtubules and discharged peroxidase-negative (specific) but not peroxidase-position (azurophile) granules. Release of lysozyme from specific granules was dose and time dependent, could be inhibitied by alpha-methyl-D-mannoside, and enhanced by cytochalasin B. Many microtubules were associated with internalized plasma membrane bearing Con A binding sites.
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