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The Journal of Cell Biology, Vol 69, 205-210, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Carbamycholine prevents giant granule-formation in cultured fibroblasts from beige (Chediak-Higashi) mice

JM Oliver, JA Krawiec and RD Berlin

Primary embryonic fibroblasts isolated from beige (Chediak-Higashi) mice develop pathognomonic giant granules in vitro. Inclusion in the culture medium of carbamylcholine (carbachol) or phorbol myristate acetate (PMA) results in the generation of morphologically normally granules. Chediak-Higashi fibroblasts are highly susceptible to shape changes induced by colchicine. This abnormal property is also corrected by carbachol and PMA.
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