JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF, 635K)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallace, R. A.
Right arrow Articles by Jared, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallace, R. A.
Right arrow Articles by Jared, D. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Journal of Cell Biology, Vol 69, 345-351, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Protein incorporation by isolated amphibian oocytes. V. Specificity for vitellogenin incorporation

RA Wallace and DW Jared

Macromolecules of vitellogenin were sequestered by Xenopus laevis oocytes 20-50 times (on a molar basis) more rapidly than other proteins tested. Selectivity for vitellogenin did not appear to involve molecular size or charge. The Km for vitellogenin incorporation was at least several orders of magnitude less than that for bovine serum albumin (BSA). At concentrations less than 10 mg-ml-1, BSA did not measurably compete with vitellogenin; a slight, apparent competition observed above a BSA concentration of 10 mg-ml-1 was probably spurious. Above a concentration of 2 mg-ml-1, vitellogenin promoted BSA incorporation by about 40%. These results are consistent with the notion that vitellogenin binds to specific receptor sites on the oocyte membrane and is subsequently internalized by micropinocytosis. Other proteins, such as BSA, which do not compete with vitellogenin are most likely to be incorporated by adventitious engulfment during micropinocytosis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents