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The Journal of Cell Biology, Vol 69, 736-740, Copyright © 1976 by The Rockefeller University Press

ARTICLES

Mating-reactive membrane vesicles from cilia of Paramecium caudatum

A Kitamura and K Hiwatashi

Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types.
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