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The Journal of Cell Biology, Vol 75, 977-982, Copyright © 1977 by The Rockefeller University Press

ARTICLES

Continuation of mitosis after selective laser microbeam destruction of the centriolar region

MW Berns and SM Richardson

The centriole regions of prophase PTK2 cells were irradiated with a laser microbeam. Cells continued through mitosis normally. Ultrastructural analysis revealed either an absence of centrioles or severely damaged centrioles at the irradiated poles. Microtubules appeared to focus into pericentriolar cloud material.
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