The Journal of Cell Biology, Vol 77, 377-388, Copyright © 1978 by The Rockefeller University Press
Effect of the H-2 gene complex rates of fibroblast intercellular adhesion
PF Bartlett and M Edidin
The rate of collection of embryo fibroblast single cells by an embryo
fibroblast monlayer was realted to the H-2 haplotype of the fibroblast
monolayer. The rate was highest for the H-2s strains and lowest for the
H-2k strains with all other strains examined being intermediate. As opposed
to monolayers prepared from the A and C3H background animals, monolayers
from B10 background mice only demonstrated an H-2 haplotype dependent rate
differential after treatment with fetal calf serum or neuraminidase. The
relationship that was seen between monolayer H-2 haplotype and rate of
adhesion with embryonic monolayers was not observed with either congenic
3T3 cell lines or fibroblasts derived from adult tissues. It was further
shown that the rate of single cell pick-up could be substantially reduced
by incubating the monolayers with the appropriate polyspecific anti-H-2
antisera. The inhibition observed appeared to be directly related to
anti-H-2 antibody binding and was not merely a function of ligand binding
to the cell surface, as antisera directed against other fibroblast cell
surface antigens did not significantly inhibit the adhesive rate. These
results indicate a role for the H-2 gene complex in modulating
fibroblast-fibroblast intercellular adhesion.
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