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The Journal of Cell Biology, Vol 79, 281-285, Copyright © 1978 by The Rockefeller University Press

ARTICLES

Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels

WS Adair, D Jurivich and UW Goodenough

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
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