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The Journal of Cell Biology, Vol 79, 617-622, Copyright © 1978 by The Rockefeller University Press

ARTICLES

Binding of [125I] wheat germ agglutinin to Chinese hamster ovary cells under conditions which affect the mobility of membrane components

P Stanley and JP Carver

The binding of [125I]wheat germ agglutinin ([125I]WGA) of high specific activity to Chinese hamster ovary (CHO) cells has been examined over a millionfold range of WGA concentrations and correlated with the phenomena of agglutination and capping by WGA. Analysis of the binding data by the method of Scatchard gives a complex curve indicative of positive cooperativity amongst high-affinity binding sites. Binding assays performed under conditions which inhibit capping and/or agglutination, such as low temperature or glutaraldehyde fixation, give similarly complex binding curves. Thus, the gross mobility of WGA receptors in the membrane does not appear to be responsible for the cooperative binding of WGA to CHO cells.
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