ARTICLE

STUDIES ON SULFHYDRYL GROUPS DURING CELL DIVISION OF SEA URCHIN EGG : II. Mass Isolation of the Egg Cortex and Change in Its—SH Groups during Cell Division

Hikoichi Sakai Ph.D.¹

¹ From the Department of Biology, Tokyo Metropolitan University, Tokyo, and the Misaki Marine Biological Station, Miura-shi, Japan

Masses of cortices of both unfertilized and fertilized sea urchin eggs can be isolated by crushing eggs in hypotonic MaCl₂ (0.1 M) solution. The amount of cortical material in terms of protein-N increases steadily after fertilization until the monaster stage and thereafter remains almost constant until well into the two-cell stage. The amount of bound—SH per protein-N of the egg cortex also increases after fertilization, reaches a maximum value at the amphiaster stage and thereafter decreases rapidly as the cleavage of the cell proceeds.

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