The Journal of Cell Biology, Vol 80, 277-290, Copyright © 1979 by The Rockefeller University Press
Mechanisms of transcription in nucleoli of amphibian oocytes as visualized by high-resolution autoradiography
N Angelier, D Hemon and M Bouteille
In oocytes of Pleurodeles waltlii, the method of Miller and Beatty has been
combined with a method of high-resolution autoradiography especially
suitable for the study of isolated molecules. In vitro labeling of RNA by
tritiated precursors was carried out with increasing incubation times (1,
4, 15, 24, 48, and 72 h). Silver grains were present over ribonucleoprotein
fibrils in amounts sufficient for quantitative analysis of nucleolar DNA
transcription. Statistical analysis of the data revealed that: (a) The
units of any one nucleolus exhibited a large degree of heterogeneity in
their number of grains. (b) There was a parallelism between the increasing
grain number and the ribonucleoprotein-fibril lengthening as observed along
the transcription unit.
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