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The Journal of Cell Biology, Vol 81, 260-265, Copyright © 1979 by The Rockefeller University Press

ARTICLES

Stereo electron microscopy of the 25-nm chromatin fibers in isolated nuclei

AL Olins and DE Olins

Thin sections (0.1-0.25 micron) of isolated chicken erythrocyte nuclei were examined at various tilt angles. Stereo pairs of electron micrographs document the parallel alignment of 25-nm chromatin fibers adjacent to the nuclear envelope, and demonstrate a fiber substructure consistent with close-packed arrays of nucleosomes.
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