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The Journal of Cell Biology, Vol 81, 680-683, Copyright © 1979 by The Rockefeller University Press

ARTICLES

A precursor of cytoplasmic actin in cultured Drosophila cells

E Berger and G Cox

In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
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