The Journal of Cell Biology, Vol 92, 665-673, Copyright © 1982 by The Rockefeller University Press
Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization
EB Lane
The tonofilament-associated protein antigens recognized in epithelial cells
by a group of six monoclonal antibodies have been studied by
immunofluorescence and gel immunoautoradiography. The monoclonal antibodies
were generated against detergent insoluble cytoskeleton extracts from a
cultured simple epithelium derived cell line, Ptk1 cells. They show various
tissue specificities, and while they all recognize components at the low
end of the molecular weight range for intermediate filament proteins, they
confirm that single antibody species can react with multiple polypeptides
of different molecular weights in the tonofilament complex. The monoclonal
antibodies described here demonstrate the presence of a simple epithelium
antigenic determinant associated with intermediate filaments that is not
detectable in the specialized cells of squamous and keratinizing epithelia
but can reappear in such cells after transformation.
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