The Journal of Cell Biology, Vol 93, 251-260, Copyright © 1982 by The Rockefeller University Press
Location and identification of the collagen found in the 14.5-d rat embryo visceral yolk sac
CC Clark, J Crossland, G Kaplan and A Martinez-Hernandez
The collagens associated with 14.5-d rat visceral yolk sacs were localized
and identified by a variety of procedures. Morphological examination showed
that both the visceral epithelium and mesothelium rested upon thin basement
membranes, whereas the majority of the extracellular matrix consisted of a
stroma containing occasional cells and abundant banded fibrils.
Immunohistochemistry at the electron microscope level showed that the
basement membranes specifically cross- reacted with antibodies directed
against mouse basement membrane components, whereas the stroma specifically
cross-reacted with antibodies directed against rat type I collagen.
Extractions of acellular visceral yolk sacs and subsequent analyses showed
that type I collagen components were prevalent. Furthermore, in vitro
biosynthetic studies showed only the presence of type I procollagen
components (or their conversion products) and alpha-fetoprotein. These
findings, taken together with our previous studies on the 14.5-d rat
parietal yolk sac, provide us with protein markers for studying the origin
of cells in rat parietovisceral yolk sac carcinomas.
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