Fibronectin has a dual role in locomotion and anchorage of primary chick fibroblasts and can promote entry into the division cycle

doi:10.1083/jcb.93.2.402

This Article

	Full Text (PDF, 1158K)
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Couchman, J. R.
	Articles by Smith, C. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Couchman, J. R.
	Articles by Smith, C. G.


Social Bookmarking

	What's this?

ARTICLES

Fibronectin has a dual role in locomotion and anchorage of primary chick fibroblasts and can promote entry into the division cycle

JR Couchman, DA Rees, MR Green and CG Smith

Fibronectin (FN), which is already known to be a natural factor for fibroblast spreading on substrata, has now been shown to be essential for two distinct types of adhesion with different biological functions in chick heart fibroblasts, namely adhesion directed toward locomotion and toward stationary anchorage for growth. Manipulation of culture conditions and the use of antisera of differing specificities has demonstrated that both exogenous and cell-derived FN are important in each process. The organization of the fibronectin-containing matrix differs between the two states. Immunoelectron microscopy with a colloidal gold marker reveals the presence of small membrane-associated plaques of fibronectin in motile cells with associated submembranous specialization. A fibrillar matrix containing fibronectin is dominant in nonmotile, growing fibroblasts. The development of focal adhesions for stationary anchorage can be dramatically enhanced by addition of cell-derived FN at an appropriate stage, and this promotes entry into the growth cycle. New macromolecular synthesis in addition to FN is necessary for focal adhesion development but not for locomotion.
Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Simpson-Holley, M., Colgrove, R. C., Nalepa, G., Harper, J. W., Knipe, D. M. (2005). Identification and Functional Evaluation of Cellular and Viral Factors Involved in the Alteration of Nuclear Architecture during Herpes Simplex Virus 1 Infection. J. Virol. 79: 12840-12851 [Abstract] [Full Text]
Hwang, J. J., Iyer, S. N., Li, L.-S., Claussen, R., Harrington, D. A., Stupp, S. I. (2002). Self-assembling biomaterials: Liquid crystal phases of cholesteryl oligo(L-lactic acid) and their interactions with cells. Proc. Natl. Acad. Sci. USA 99: 9662-9667 [Abstract] [Full Text]
Yamamoto, H., Oviedo, A., Sweeley, C., Saito, T., Moskal, J. R. (2001). {alpha}2,6-Sialylation of Cell-Surface N-Glycans Inhibits Glioma Formation in Vivo. Cancer Res. 61: 6822-6829 [Abstract] [Full Text]
Palecek, S., Huttenlocher, A, Horwitz, A., Lauffenburger, D. (1998). Physical and biochemical regulation of integrin release during rear detachment of migrating cells. J. Cell Sci. 111: 929-940 [Abstract]
Xie, H, Pallero, M., Gupta, K, Chang, P, Ware, M., Witke, W, Kwiatkowski, D., Lauffenburger, D., Murphy-Ullrich, J., Wells, A (1998). EGF receptor regulation of cell motility: EGF induces disassembly of focal adhesions independently of the motility-associated PLCgamma signaling pathway. J. Cell Sci. 111: 615-624 [Abstract]
Dunlevy, J., Couchman, J. (1993). Controlled induction of focal adhesion disassembly and migration in primary fibroblasts. J. Cell Sci. 105: 489-500 [Abstract]
Ingber, D. (1993). Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton. J. Cell Sci. 104: 613-627