The Journal of Cell Biology, Vol 94, 201-206, Copyright © 1982 by The Rockefeller University Press

ARTICLES

Three mutations in Zea mays affecting zein accumulation: a comparison of zein polypeptides, in vitro synthesis and processing, mRNA levels, and genomic organization

FA Burr and B Burr

We studied three mutations, opaque-2 (o2), opaque-7 (o7), and floury- 2(fI2), each of which causes a depression in zein synthesis. We examined the processing efficiencies of the rough endoplasmic reticulum membranes in vitro, the levels of RNA transcription using cloned zein probes, and the genomic organization of the zein sequences as possible sites for the genetic defects. The results obtained indicate that the steps in prezein translation and processing occurring on the protein body membranes are not accountable for the lowered zein content in any of the mutations. The o2 mutation that typically shows a paucity of 22.5-kdalton zein polypeptides was found to have a concomitant reduction in a particular subgroup of mRNAs coding for this size class. Southern analyses suggest that the o2 mutation is not the result of a large deletion of tandem-linked zein genes.
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This article has been cited by other articles:

• Aukerman, M J, Schmidt, R J, Burr, B, Burr, F A (1991). An arginine to lysine substitution in the bZIP domain of an opaque-2 mutant in maize abolishes specific DNA binding.. Genes Dev. 5: 310-320 [Abstract]  
• Schmidt, R., Burr, F., Burr, B (1987). Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science 238: 960-963 [Abstract]  

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