The Journal of Cell Biology, Vol 96, 1197-1207, Copyright © 1983 by The Rockefeller University Press
The rough endoplasmic reticulum and the Golgi apparatus visualized using specific antibodies in normal and tumoral prolactin cells in culture
C Tougard, D Louvard, R Picart and A Tixier-Vidal
Antibodies directed against membrane components of dog pancreas rough
endoplasmic reticulum (A-RER) and rat liver Golgi apparatus (A-Golgi)
(Louvard, D., H. Reggio, and G. Warren, 1982, J. Cell Biol. 92:92-107) have
been applied to cultured rat prolactin (PRL) cells, either normal cells in
primary cultures, or clonal GH3 cells. In normal PRL cells, the A-RER
stained the membranes of the perinuclear cisternae as well as those of many
parallel RER cisternae. The A-Golgi stained part of the Golgi membranes. In
the stacks it stained the medial saccules and, with a decreasing intensity,
the saccules of the trans side, as well as, in some cells, a linear
cisterna in the center of the Golgi zone. It also stained the membrane of
many small vesicles as well as that of lysosomelike structures in all
cells. In contrast, it never stained the secretory granule membrane, except
at the level of very few segregating granules on the trans face of the
Golgi zone. In GH3 cells the A-RER stained the membrane of the perinuclear
cisternae, as well as that of short discontinuous flat cisternae. The
A-Golgi stained the same components of the Golgi zone as in normal PRL
cells. In some cells of both types the A-Golgi also stained discontinuous
patches on the plasma membrane and small vesicles fusing with the plasma
membrane. Immunostaining of Golgi membranes revealed modifications of
membrane flow in relation to either acute stimulation of PRL release by
thyroliberin or inhibition of basal secretion by monensin.
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