The Journal of Cell Biology, Vol 97, 627-637, Copyright © 1983 by The Rockefeller University Press

ARTICLES

Transepithelial transport of a viral membrane glycoprotein implanted into the apical plasma membrane of Madin-Darby canine kidney cells. I. Morphological evidence

K Matlin, DF Bainton, M Pesonen, D Louvard, N Genty and K Simons

The G protein of vesicular stomatitis virus was implanted in the apical plasma membrane of Madin-Darby canine kidney cells by low pH-dependent fusion of the viral envelope with the cellular membrane. The amount of fusion as determined by removal of unfused virions, either by tryptic digestion or by EDTA treatment at 0 degree C, was 22-24% of the cell- bound virus radioactivity. Upon incubation of cells after implantation, the amount of G protein as detected by immunofluorescence diminished on the apical membrane and appeared within 30 min on the basolateral membrane. At the same time some G protein fluorescence was also seen in intracellular vacuoles. The observations by immunofluorescence were confirmed and extended by electron microscopy. Using immunoperoxidase localization, G protein was seen to move into irregularly shaped vacuoles (endosomes) and multivesicular bodies and to appear on the basolateral plasma membrane. These results suggest that the apical and basolateral domains of Madin-Darby canine kidney cells are connected by an intracellular route.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Carstanjen, D., Dutt, P., Moritz, T. (2001). Heparin Inhibits Retrovirus Binding to Fibronectin as Well as Retrovirus Gene Transfer on Fibronectin Fragments. J. Virol. 75: 6218-6222 [Abstract] [Full Text]  
• Charron, A. J., Nakamura, S., Bacallao, R., Wandinger-Ness, A. (2000). Compromised Cytoarchitecture and Polarized Trafficking in Autosomal Dominant Polycystic Kidney Disease Cells. JCB 149: 111-124 [Abstract] [Full Text]  
• Jacob, R., Alfalah, M., Grunberg, J., Obendorf, M., Naim, H. Y. (2000). Structural Determinants Required for Apical Sorting of an Intestinal Brush-border Membrane Protein. J. Biol. Chem. 275: 6566-6572 [Abstract] [Full Text]  
• Rahkila, P., Luukela, V., Vaananen, K., Metsikko, K. (1998). Differential Targeting of Vesicular Stomatitis Virus G Protein and Influenza Virus Hemagglutinin Appears During Myogenesis of L6 Muscle Cells. JCB 140: 1101-1111 [Abstract] [Full Text]  
• Palokangas, H, Mulari, M, Vaananen, H. (1997). Endocytic pathway from the basal plasma membrane to the ruffled border membrane in bone-resorbing osteoclasts. J. Cell Sci. 110: 1767-1780 [Abstract]  
• Rahkila, P, Alakangas, A, Vaananen, K, Metsikko, K (1996). Transport pathway, maturation, and targetting of the vesicular stomatitis virus glycoprotein in skeletal muscle fibers. J. Cell Sci. 109: 1585-1596 [Abstract]  
• Sariola, M, Saraste, J, Kuismanen, E (1995). Communication of post-Golgi elements with early endocytic pathway: regulation of endoproteolytic cleavage of Semliki Forest virus p62 precursor. J. Cell Sci. 108: 2465-2475 [Abstract]  
• Nelson, W.J., Wilson, R., Wollner, D., Mays, R., McNeill, H., Siemers, K. (1992). Regulation of Epithelial Cell Polarity: A View from the Cell Surface. Cold Spring Harb Symp Quant Biol 57: 621-630 [Abstract]  
• Rodriguez-Boulan, E, Nelson, W. (1989). Morphogenesis of the polarized epithelial cell phenotype. Science 245: 718-725 [Abstract]  
• Spodsberg, N., Jacob, R., Alfalah, M., Zimmer, K.-P., Naim, H. Y. (2001). Molecular Basis of Aberrant Apical Protein Transport in an Intestinal Enzyme Disorder. J. Biol. Chem. 276: 23506-23510 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents