Val-Gly-Val-Ala-Pro-Gly, a repeating peptide in elastin, is chemotactic for fibroblasts and monocytes

doi:10.1083/jcb.99.3.870

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Val-Gly-Val-Ala-Pro-Gly, a repeating peptide in elastin, is chemotactic for fibroblasts and monocytes

RM Senior, GL Griffin, RP Mecham, DS Wrenn, KU Prasad and DW Urry

Recent studies have demonstrated that tropoelastin and elastin-derived peptides are chemotactic for fibroblasts and monocytes. To identify the chemotactic sites on elastin, we examined the chemotactic activity of Val-Gly-Val-Ala-Pro-Gly (VGVAPG), a repeating peptide in tropoelastin. We observed that VGVAPG was chemotactic for fibroblasts and monocytes, with optimal activity at approximately 10(-8) M, and that the chemotactic activity of VGVAPG was substantial (half or greater) relative to the maximum responses to other chemotactic factors such as platelet-derived growth factor for fibroblasts and formyl-methionyl- leucyl-phenylalanine for monocytes. The possibility that at least part of the chemotactic activity in tropoelastin and elastin peptides is contained in VGVAPG sequences was supported by the following: (a) polyclonal antibody to bovine elastin selectively blocked the fibroblast and monocyte chemotactic activity of both elastin-derived peptides and VGVAPG; (b) monocyte chemotaxis to VGVAPG was selectively blocked by preexposing the cells to elastin peptides; and (c) undifferentiated (nonelastin producing) bovine ligament fibroblasts, capable of chemotaxis to platelet-derived growth factor, did not show chemotactic responsiveness to either VGVAPG or elastin peptides until after matrix-induced differentiation and the onset of elastin synthesis. These studies suggest that small synthetic peptides may be able to reproduce the chemotactic activity associated with elastin- derived peptides and tropoelastin.
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Duca, L., Blanchevoye, C., Cantarelli, B., Ghoneim, C., Dedieu, S., Delacoux, F., Hornebeck, W., Hinek, A., Martiny, L., Debelle, L. (2007). The Elastin Receptor Complex Transduces Signals through the Catalytic Activity of Its Neu-1 Subunit. J. Biol. Chem. 282: 12484-12491 [Abstract] [Full Text]
Guo, G., Booms, P., Halushka, M., Dietz, H. C., Ney, A., Stricker, S., Hecht, J., Mundlos, S., Robinson, P. N. (2006). Induction of Macrophage Chemotaxis by Aortic Extracts of the mgR Marfan Mouse Model and a GxxPG-Containing Fibrillin-1 Fragment. Circulation 114: 1855-1862 [Abstract] [Full Text]
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Cantor, J. O., Shteyngart, B., Cerreta, J. M., Turino, G. M. (2002). The Effect of Lysozyme on Elastase-Mediated Injury. Exp. Biol. Med. 227: 108-113 [Abstract] [Full Text]
Frisdal, E., Gest, V., Vieillard-Baron, A., Levame, M., Lepetit, H., Eddahibi, S., Lafuma, C., Harf, A., Adnot, S., d'Ortho, M-;P. (2001). Gelatinase expression in pulmonary arteries during experimental pulmonary hypertension. Eur Respir J 18: 838-845 [Abstract] [Full Text]
Cai, G.-Z., Griffin, G. L., Senior, R. M., Longmore, W. J., Moxley, M. A. (1999). Recombinant SP-D carbohydrate recognition domain is a chemoattractant for human neutrophils. Am. J. Physiol. Lung Cell. Mol. Physiol. 276: L131-L136 [Abstract] [Full Text]
Faury, G., Garnier, S., Weiss, A. S., Wallach, J., Fulop, T. Jr, Jacob, M.-P., Mecham, R. P., Robert, L., Verdetti, J. (1998). Action of Tropoelastin and Synthetic Elastin Sequences on Vascular Tone and on Free Ca2+ Level in Human Vascular Endothelial Cells. Circ. Res. 82: 328-336 [Abstract] [Full Text]
Dunlevy, J., Couchman, J. (1995). Interleukin-8 induces motile behavior and loss of focal adhesions in primary fibroblasts. J. Cell Sci. 108: 311-321 [Abstract]
Chello, M., Mastroroberto, P., Romano, R., Cirillo, F., Cusano, T., Marchese, A. R. (1994). Alteration in Collagen and Elastin Content in Varicose Veins. VASC ENDOVASCULAR SURG 28: 23-27 [Abstract]
Dunlevy, J., Couchman, J. (1993). Controlled induction of focal adhesion disassembly and migration in primary fibroblasts. J. Cell Sci. 105: 489-500 [Abstract]
Hinek, A, Wrenn, D., Mecham, R., Barondes, S. (1988). The elastin receptor: a galactoside-binding protein. Science 239: 1539-1541 [Abstract]
Brassart, B., Fuchs, P., Huet, E., Alix, A. J. P., Wallach, J., Tamburro, A. M., Delacoux, F., Haye, B., Emonard, H., Hornebeck, W., Debelle, L. (2001). Conformational Dependence of Collagenase (Matrix Metalloproteinase-1) Up-regulation by Elastin Peptides in Cultured Fibroblasts. J. Biol. Chem. 276: 5222-5227 [Abstract] [Full Text]