The Journal of Cell Biology, Vol 99, 1451-1460, Copyright © 1984 by The Rockefeller University Press
Distribution of epinemin in colloidal gold-labelled, quick-frozen, deep- etched cytoskeletons
D Lawson
In this study I describe the ultrastructural distribution of epinemin
(Lawson, D., 1983, J. Cell Biol., 97:1891-1905) in antibody-labelled,
helium-cooled, quick-frozen, deep-etched cytoskeletons. This technique
reveals that epinemin is expressed asymmetrically at discrete sites on the
vimentin core polymer and that usually one (occasionally two or three)
antiepinemin molecules are found at each of these discrete foci. Single
receptor-bound antiepinemin (IgM) molecules are easily identified in
deep-etched cytoskeletons by the use of colloidal gold. Epinemin does not
cross-link adjacent intermediate filaments and is not associated with the
many 2-3-nm filaments found associated with intermediate filaments in these
preparations. The directional changes and interactions undergone by
microtubules in taxol-stabilized, antibody-labelled cytoskeletons are also
discussed.
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