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Published online May 12, 2008
doi:10.1083/jcb.200803027
The Journal of Cell Biology
The Rockefeller University Press, 0021-9525 $30.00
© 2008 Joglekar et al.
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 Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres

Ajit P. Joglekar1, David Bouck2, Ken Finley3, Xingkun Liu4, Yakun Wan5, Judith Berman3, Xiangwei He4, E.D. Salmon1, and Kerry S. Bloom1

1 Department of Biology, University of North Carolina, Chapel Hill, NC 27599
2 St. Jude Children's Research Hospital, Memphis, TN 38105
3 Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455
4 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030
5 Institute for Systems Biology, Seattle, WA 98103

Correspondence to Kerry S. Bloom: kbloom{at}email.unc.edu

Point and regional centromeres specify a unique site on each chromosome for kinetochore assembly. The point centromere in budding yeast is a unique 150-bp DNA sequence, which supports a kinetochore with only one microtubule attachment. In contrast, regional centromeres are complex in architecture, can be up to 5 Mb in length, and typically support many kinetochore-microtubule attachments. We used quantitative fluorescence microscopy to count the number of core structural kinetochore protein complexes at the regional centromeres in fission yeast and Candida albicans. We find that the number of CENP-A nucleosomes at these centromeres reflects the number of kinetochore-microtubule attachments instead of their length. The numbers of kinetochore protein complexes per microtubule attachment are nearly identical to the numbers in a budding yeast kinetochore. These findings reveal that kinetochores with multiple microtubule attachments are mainly built by repeating a conserved structural subunit that is equivalent to a single microtubule attachment site.


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