Correction

An incorrect graphic appears in the legend for Fig. 1. The corrected sentence appears below in bold.

Figure 1. A two-step subcellular fractionation procedure for separation of late endosomes and DCG. (A) Distribution of intracellular compartments from PC12 cells on 1–16% Ficoll gradients. PC12 cells expressing ssHRP^P-selectin were loaded with ³H-Dopamine, or labeled with ¹²⁵I-Trn or ¹²⁵I-EGF as described in Materials and Methods. Cells were homogenized in HB and the PNS was centrifuged on 1–16% Ficoll gradients and fractionated. The early/recycling endosomes are shown by the distribution of ¹²⁵I-Trn endocytosed for 60 min at 37°C (). Late endosomes are shown by the distribution of ¹²⁵I-EGF internalized for 20 min at 37°C (). The distribution of NAGA activity (OD_{420 nm}; ), HRP activity (OD_{450 nm}; •), and ³H-Dopamine radioactivity (filled plus signs) along 1–16% Ficoll gradients are shown. (B) Separation of DCG and late endosomes on a secondary 0.9–1.85 M sucrose gradient. Fractions 14–20 from the 1–16% Ficoll gradients were pooled, diluted with HB, and recentrifuged on 0.9–1.85 M sucrose gradients to equilibrium. The distributions of NAGA activity (), HRP activity (•), ³H-Dopamine radioactivity (filled plus signs), ¹²⁵I-EGF internalized for 20 min at 37°C () and ¹²⁵I-Trn () after centrifugation on this gradient are shown.

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