Original Article

The Actin-Driven Movement and Formation of Acetylcholine Receptor Clusters

Correspondence to: H. Benjamin Peng, Department of Cell Biology and Anatomy, University of North Carolina, CB#7090, 108 Taylor Hall, Chapel Hill, NC 27599-7090. Tel:(919) 843-7185 Fax:(919) 966-1856

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

A new method was devised to visualize actin polymerization induced by postsynaptic differentiation signals in cultured muscle cells. This entails masking myofibrillar filamentous (F)-actin with jasplakinolide, a cell-permeant F-actin–binding toxin, before synaptogenic stimulation, and then probing new actin assembly with fluorescent phalloidin. With this procedure, actin polymerization associated with newly induced acetylcholine receptor (AChR) clustering by heparin-binding growth-associated molecule–coated beads and by agrin was observed. The beads induced local F-actin assembly that colocalized with AChR clusters at bead–muscle contacts, whereas both the actin cytoskeleton and AChR clusters induced by bath agrin application were diffuse. By expressing a green fluorescent protein–coupled version of cortactin, a protein that binds to active F-actin, the dynamic nature of the actin cytoskeleton associated with new AChR clusters was revealed. In fact, the motive force generated by actin polymerization propelled the entire bead-induced AChR cluster with its attached bead to move in the plane of the membrane. In addition, actin polymerization is also necessary for the formation of both bead and agrin-induced AChR clusters as well as phosphotyrosine accumulation, as shown by their blockage by latrunculin A, a toxin that sequesters globular (G)-actin and prevents F-actin assembly. These results show that actin polymerization induced by synaptogenic signals is necessary for the movement and formation of AChR clusters and implicate a role of F-actin as a postsynaptic scaffold for the assembly of structural and signaling molecules in neuromuscular junction formation.

Key Words: acetylcholine receptor cluster, actin, neuromuscular junction, latrunculin, jasplakinolide

	Introduction

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

Innervation of the skeletal muscle signals differentiation of the sarcolemma at sites of nerve–muscle contact. This leads to the formation of acetylcholine receptor (AChR)¹ clusters at the postsynaptic membrane. These receptors are initially distributed in a diffuse manner in the muscle membrane and become clustered to extremely high density during the formation of the neuromuscular junction (NMJ; Sanes and Lichtman 1999 ). The signal transduction mechanism involved in AChR clustering during NMJ formation has been extensively studied both in vitro and in vivo. Agrin, a heparan-sulfate proteoglycan, is released by the motoneuron growth cone to induce postsynaptic differentiation (Bowe and Fallon 1995 ; Ruegg and Bixby 1998 ; Gautam et al. 1996 ). Recent works from our laboratory have also suggested the involvement of heparan-sulfate proteoglycan–bound growth factors in the signaling mechanism (Rauvala and Peng 1997 ; Daggett et al. 1996a ). Agrin, together with other yet to be unidentified muscle-derived signals, appears to activate the receptor tyrosine kinase (RTK) MuSK (muscle-specific kinase) to initiate the signal transduction cascade (Sanes and Lichtman 1999 ; Ganju et al. 1995 ; Glass et al. 1996 ). Steps following MuSK activation are largely unknown. Recent studies have shown that MuSK, similar to other RTKs, can be activated by dimerization (Hopf and Hoch 1998 ; Xie et al. 1997 ). A recurring theme in signal transduction involving RTKs is that their activation leads to the assembly of a cortical filamentous actin (F-actin) cytoskeleton through highly regulated polymerization of globular actin (G-actin) monomers (Rijken et al. 1991 , Rijken et al. 1995 ; Peppelenbosch et al. 1993 ). This event is critical to RTK-mediated signaling, as shown by the fact that blocking F-actin assembly by cytochalasins causes abolishment of subsequent steps in the signaling cascade (Ojaniemi and Vuori 1997 ; Rijken et al. 1998 ; Tapia et al. 1999 ). Thus, the F-actin cytoskeleton serves as a scaffold for the assembly of the signaling machinery.

An F-actin cytoskeleton is associated with the postsynaptic membrane at the NMJ and at AChR clusters in cultured muscle cells (Connolly 1984 ; Bloch 1986 ; Peng and Phelan 1984 ). It is generally believed that this cytoskeleton is involved in the formation and stabilization of AChR clusters. However, because of the abundance of myofibrillar actin, it has been difficult to demonstrate actin polymerization associated with synaptogenic stimulation. Furthermore, due to the ineffectiveness of conventional blockers for actin polymerization such as cytochalasins in disrupting AChR clusters (Connolly 1984 ; Moody-Corbett and Cohen 1982a ), the necessity of F-actin assembly in the formation of AChR clusters has not been established.

In this study, a novel labeling procedure was devised to examine actin polymerization at developing AChR clusters. This method is based on the use of jasplakinolide to mask preexisting F-actin structures. Jasplakinolide is a marine sponge toxin that resembles the mushroom toxin phalloidin in its highly specific binding to F-actin but is much more cell-permeant (Crews et al. 1986 ; Bubb et al. 1994 ; Zabriskie et al. 1999 ). This procedure enabled unprecedented clarity in detecting newly assembled F-actin cytoskeleton at developing AChR clusters in cultured muscle cells. We found that actin polymerization at newly formed AChR clusters can generate enough force to propel the entire AChR complex to move in the plane of the membrane. With latrunculin A (Ltn A), another marine sponge toxin that binds to G-actin with 1:1 stoichiometry and inhibits its polymerization (Spector et al. 1983 ; Coue et al. 1987 ; Morton et al. 2000 ), we also established the necessity of actin polymerization in AChR cluster formation and its accompanied accumulation of phosphotyrosine (PY). These data ascribe a pivotal role of actin polymerization in the assembly of AChR clusters and in providing a driving force for the novel motility of the transmembrane AChR protein complex within sarcolemma.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

Materials
Jasplakinolide, rhodamine-conjugated phalloidin (Rh-phalloidin), and rhodamine-conjugated -bungarotoxin (R-BTX) were purchased from Molecular Probes. Oregon green–conjugated BTX (OG-BTX) was a gift from Dr. Richard Rotundo (University of Miami, Miami, FL). Ltn A was purchased from Molecular Probes and from Biomol. Monoclonal anti-PY antibody 4G10 was purchased from Upstate Biotechnology. Fluorescence-conjugated secondary antibodies were obtained from Organon-Teknika Cappel. Recombinant heparin-binding growth-associated molecule (HB-GAM), kindly provided by Dr. Hekki Rauvala (University of Helsinki, Helsinki, Finland), was produced in SF9 cells infected with baculovirus carrying rat HB-GAM coding sequence. The factor was purified to homogeneity from the culture medium as previously described (Raulo et al. 1992 ).

Cell Culture and AChR Cluster Induction
Muscle cells were isolated from Xenopus embryos according to a previously published method (Peng et al. 1991 ). They were plated on glass coverslips and cultured in Steinberg medium (60 mM NaCl, 0.7 mM KCl, 0.4 mM Ca(NO₃)₂, 0.8 mM MgSO₄, 10 mM Hepes, pH 7.4, plus 10% L-15, 1% fetal bovine medium, and 0.1 mg/ml gentamycin). The cultures were maintained at 22°C for 24 h and then stored in a 15°C incubator before being used for experiments.

AChR cluster formation was induced by treating muscle cultures either with agrin or with HB-GAM–coated beads. Recombinant agrin was made by transfecting HEK 293 cells with a cDNA encoding the A4B8 form of chick agrin (Daggett et al. 1996b ). Conditioned medium from transfected cells was used in this study. Muscle cultures were treated with the conditioned medium at 1:10 dilution and AChR clustering was examined after an overnight incubation. Induction of AChR clustering by beads was carried out according to our previously published method (Peng et al. 1995 ). 5- or 10-µm-diameter polystyrene microspheres (Polysciences, Inc.) were coated with recombinant HB-GAM and applied to muscle cultures for a duration ranging from several hours to overnight. In both agrin and bead-stimulated cultures, AChR clusters were visualized by labeling with 300 nM R-BTX for 30 min. Cells were imaged with a Leitz Orthoplan microscope with an x63 objective (NA 1.4) using Hamamatsu C5985 or ORCA II cooled CCD camera and Metamorph software (Universal Imaging) either live or after fixation with 95% ethanol at -20°C.

To visualize PY associated with AChR clusters, ethanol-fixed cultures were labeled with mAb 4G10 followed by FITC-conjugated secondary antibody according to our previously published procedure (Baker and Peng 1993 ).

Ltn A and Jasplakinolide Application and F-actin Visualization
Ltn A and jasplakinolide were dissolved in DMSO to make the stock solution at 1 mM concentration and stored frozen. Aliquots of the frozen stock were serially diluted into culture medium to make the experimental solution. To examine actin polymerization associated with AChR clustering, muscle cultures were pretreated with jasplakinolide at a concentration of 2.5 or 10 µM for 3 h and then rinsed with culture medium before agrin or beads were applied. The assembly of the actin cytoskeleton was visualized by labeling ethanol fixed cells with Rh-phalloidin (1:500 dilution from the stock of 200 U/ml) for 30 min. To study the effects of Ltn A and jasplakinolide on AChR clustering, muscle cultures were preincubated with Ltn A or jasplakinolide for 30 min. Agrin or bead stimuli were then introduced. After an incubation period ranging from 6 to 24 h in the continuous presence of the toxin, the culture was labeled with R-BTX and fixed for imaging.

Expression of Cortactin–Green Fluorescent Protein in Cultured Muscle Cells
Mouse cortactin cDNA (kindly provided by Dr. Tom Parsons, University of Virginia, Charlottesville, VA) was subcloned into pEGFP-N1 vector (CLONTECH Laboratories, Inc.) and verified by sequencing (kindly provided by M. Kaksonen, University of Helsinki). The cortactin-EGFP cassette was then subcloned into pCS2+ vector in polylinker 1 region downstream from SP6 promoter. To make synthetic mRNA of the cortactin-EGFP chimeric protein, the plasmid was linearized and transcribed with SP6 RNA polymerase using a kit from Ambion, Inc., following the manufacturer's instructions. The mRNA was injected into blastomeres at 1–2-cell stage with a Drummond oocyte injector (Drummond Scientific Co.). 1 to 2 ng mRNA in 4.6 to 9.2 nl was injected into each embryo. Embryos that showed green fluorescent protein (GFP) expression were used to prepare muscle cultures. Cultured muscle cells identified by GFP fluorescence were examined for the localization of the chimeric protein after agrin or bead treatment.

	Results

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

Induction of Actin Polymerization by Synaptogenic Signals
The F-actin localization at AChR clusters was examined by labeling muscle cells with Rh-phalloidin. The abundance of myofibrillar F-actin in skeletal muscle cells made detection of its localization at AChR clusters difficult except at thin, lamellar areas. As shown in Fig 2A and Fig B (see below), F-actin was concentrated at spontaneously formed AChR hot spots. Despite their colocalization, the F-actin patch was not congruent with the AChR cluster and they often existed in nearly complementary patterns.

View larger version (75K): [in this window] [in a new window]   Figure 1. Masking of preexistent F-actin structures by jasplakinolide pretreatment. (A–C) Competition between jasplakinolide and phalloidin for F-actin binding. (A) Rh-phalloidin labeling of myofibrils. (B and C) Pretreating the culture with 1 µM jasplakinolide followed by Rh-phalloidin blocked myofibril labeling, thus showing that these two toxins compete for F-actin binding. (D–G) Jasplakinolide pretreatment does not abolish new F-actin assembly in motile muscle cells. This cell was treated with 10 µM jasplakinolide for 3 h and rinsed with drug-free medium. The lamellipodia developed after jasplakinolide removal (compare D and E) exhibited F-actin assembly as shown by Rh-phalloidin labeling (G) at the end of the 24-h period. D and E are live images and F and G are fixed cell images. Phase–contrast of the cell at 24 h is shown in F. (H–K) In contrast to motile cells, this nonmotile cell showed no new F-actin assembly after jasplakinolide pretreatment. H and I are live images and J and K are fixed cell images.

View larger version (73K): [in this window] [in a new window]   Figure 2. F-actin assembly at AChR clusters induced by HB-GAM beads. A, C, F, and I, Rh-phalloidin labeling; B, D, G, and J, OG-BTX labeling. (A and B) F-actin at an AChR hot spot in a thin lamella region. Phalloidin-labeled myofibrils and F-actin at AChR cluster. (C, D, and F–J) Jasplakinolide pretreatment to mask preexisting F-actin before bead stimulation. The beads induced spatially discrete actin polymerization and AChR clustering as shown in two examples (C and D and F–H). In addition to sites of bead–muscle contacts, new F-actin assembly was also seen at filopodia and lamellipodia at the cell periphery (F, arrows). H is a phase image of the cell shown in F and G. At higher magnification (I and J), the punctate and circumferential pattern of new F-actin can be seen. The F-actin and AChR patterns are not congruent. (E) Quantification of F-actin and AChR induction by beads. The majority of beads induced both specializations (correlation coefficient 0.987). Data from 40 cells are pooled.

To examine new F-actin assembly induced by AChR clustering stimuli, we experimented with jasplakinolide to mask the myofibrillar phalloidin labeling. This marine sponge toxin binds to F-actin with high specificity and is cell permeant. It competes with phalloidin for F-actin binding as shown in Fig 1 (A–C). After pretreating live muscle cells with micromolar concentrations of jasplakinolide, subsequent rhodamine-phalloidin labeling of preexisting F-actin structures, most notably myofibrils and AChR hot spots, was greatly diminished. However, this treatment did not prevent new actin polymerization associated with muscle cell motility such as lamellipodial extension as in the example shown in Fig 1D–G. In contrast, cells which did not exhibit such motility showed little new actin assembly (Fig 1, H–K). This demonstrates the utility of this jasplakinolide application in masking preexisting F-actin to allow visualization of new actin assembly in muscle cells. Thus, this procedure was applied to examine actin polymerization induced by two stimuli known to induce AChR clustering. Latex beads coated with HB-GAM have been shown to induce discrete AChR clusters at bead-muscle contact sites in cultured Xenopus muscle cells (Peng et al. 1995 ). On the other hand, bath application of agrin, in particular the neuronal A4B8 form containing inserts of 4 and 8 amino acids at A and B positions, respectively, induces AChR clustering in a diffuse manner over the entire Xenopus muscle cell (Daggett et al. 1996b ).

After masking preexistent F-actin by treating cells with 2.5–10 µM jasplakinolide for 3 h followed by extensive rinsing, the stimulus (beads or agrin) was applied, and 24 h later new F-actin was labeled by Rh-phalloidin and new AChR clusters were detected by OG-BTX. As shown in Fig 2 (C–D and F–H), F-actin assembly was discretely localized at bead-induced AChR clusters. In the second example (Fig 2, F–H), new actin polymerization was seen at bead-induced AChR clusters as well as at sites of lamellipodial and filopodial activity at the periphery of this muscle cell. At higher magnification, one can see that F-actin induced by beads existed in two configurations: a punctate pattern in the central region of the bead–muscle contact and a circumferential filament bundle surrounding the central region (Fig 2, I–J). Interestingly, bead-induced AChR clusters also conformed to these two F-actin domains (Fig 2D, Fig G, and Fig J). However, the F-actin and AChR patterns were not congruent with each other. The majority of beads that induced AChR clusters also generated F-actin assembly (Fig 2 E). Only rarely did we observe beads with only one type of specialization but not the other. In addition, no statistically significant difference was seen in the percentage of beads that induced AChR clustering after jasplakinolide pretreatment as compared with control cultures (percentage of beads with AChR clusters = 86 ± 2% after pretreatment versus 85 ± 2% in control).

On the other hand, bath application of agrin induced a more diffuse F-actin assembly and AChR clustering (Fig 3). In general, F-actin patches induced by agrin were not closely associated with AChR clusters (Fig 3, C–D). However, good colocalization of these two specializations was also detectable among clusters in an unpredictable manner as shown in Fig 3 A and B (circles). Even in this case, a lack of congruency between F-actin and AChR clustering patterns is obvious. Similar to the bead-stimulated process, jasplakinolide pretreatment did not affect the number of agrin-induced clusters as compared with control cultures.

View larger version (82K): [in this window] [in a new window]   Figure 3. F-actin assembly induced by agrin after jasplakinolide pretreatment. Unlike beads, agrin induced a more diffuse F-actin assembly which paralleled the scattered AChR clustering pattern. (A and B) Examples of colocalization of agrin-induced F-actin and AChR patches (circles). However, the patterns of these two specializations are different despite their colocalization. (C and D) An example of the general lack of precise registration in these two specializations.

These data show that synaptogenic stimuli such as HB-GAM–coated beads and agrin induce the assembly of new F-actin. In contrast, the lack of phalloidin labeling at hot spots after jasplakinolide pretreatment indicates an absence of new F-actin assembly at these preexisting AChR clusters.

Cortactin–EGFP Localization at Newly Formed AChR Clusters
F-actin structures such as myofibrils in muscle cells are stable and undergo relatively little elongation and shrinkage (Littlefield and Fowler 1998 ). In contrast, F-actin meshwork within the leading edge of motile cells is highly dynamic and retains polymerization and depolymerization activities (Condeelis 1993 ). Cortactin is an F-actin binding protein that binds differentially to active F-actin meshwork at the leading edge and thus can be used as a marker to reveal the dynamic nature of its assembly (Wu and Parsons 1993 ). To assess the extent of actin polymerization within the cytoskeleton at newly formed AChR clusters, we expressed cortactin–EGFP in cultured muscle cells and examined its distribution in relationship to receptor clusters induced by beads and agrin.

In quiescent cells, cortactin-EGFP was concentrated at lamellar structures that are present predominantly at the ends of muscle cells (Fig 4 A). AChR hot spots generally were not associated with cortactin–EGFP (Fig 4A and Fig B). In situations when it was detected at hot spots, the cortactin patch was usually smaller than the AChR cluster (data not shown). After HB-GAM bead treatment, cortactin–EGFP became reliably associated with bead-induced AChR clusters (Fig 4C and Fig D, arrows), although they were not congruent with each other. In response to bath agrin application, cortactin–EGFP also became membrane associated (Fig 4 E). However, unlike bead-treated cells, agrin-induced cortactin distribution was more diffuse. Although cortactin–EGFP was not precisely colocalized with agrin-induced AChR clusters, a general coexistence of these two molecules could be observed (Fig 4E and Fig F). AChR clusters were more likely to be found along cortactin–EGFP enriched membrane areas.

View larger version (69K): [in this window] [in a new window]   Figure 4. Cortactin-EGFP expression. (A and B) In unstimulated cells, cortactin was concentrated at membrane lamellae and often showed no colocalized with AChR hot spots. (C and D) Bead-induced AChR clusters were marked by cortactin localization. (E and F) Agrin induced association of cortactin with membrane areas encompassing AChR clusters.

Thus, HB-GAM beads induce a discrete localization of cortactin, whereas agrin induces a more diffuse membrane association of this protein. These two contrasting patterns of cortactin distribution are in agreement with those of F-actin distribution described above. The cortactin localization suggests an ongoing, dynamic F-actin assembly induced by beads and agrin.

Movement of AChR Clusters Propelled by Actin Polymerization
We next sought to understand the function of the F-actin assembly induced by AChR clustering stimuli. Sustained actin polymerization has been shown to be the mechanism of generating plasma membrane protrusions such as lamellipodia and filopodia (Condeelis 1993 ). It also provides motile force for certain intracellular pathogens such as Listeria and Vaccinia to move in the cytoplasm of infected cells (Theriot et al. 1992 ; Cudmore et al. 1995 ). We thus examined whether newly formed AChR clusters induced by beads could undergo movement. AChR clusters were first induced by 5-µm HB-GAM beads. 24 h after bead treatment, when the clusters were well formed as shown by their bright R-BTX labeling, they were followed by fluorescence microscopy under low-light illumination.

As shown in Fig 5, AChR clusters underwent displacement on the cell surface over a distance of 10 µm during the 40-h recording period. During this period of time, there was little cell movement as shown by (i) the constant distance between markers on and outside the cell (arrows in Fig 5 B) and (ii) neighboring clusters often moved independently in both direction and velocity despite their proximity to each other (compare clusters 1 and 2 with 3 in Fig 5 A and beads 3 and 4 in Fig 6A and Fig C). Corresponding phase–contrast images showed that beads always moved with their associated AChR clusters as one unit. Thus, bead tracking offered a simple and less invasive means to study the cluster movement. As shown in Fig 6A and Fig B, four bead-associated AChR clusters were followed for 24 h by recording bead positions at 10-min intervals. Their trajectories are plotted in Fig 6 C. The beads moved in either a directional or zigzagged manner for a distance of 10 µm during the recording period. To study the role of actin polymerization in this AChR cluster movement, the bead movement was followed in the presence of Ltn A, which sequesters G-actin and prevents its polymerization into F-actin (Spector et al. 1983 ; Coue et al. 1987 ). As shown in Fig 6 D, except for a small degree of diffusional drift (see below), the bead movement was completely abolished.

View larger version (38K): [in this window] [in a new window]   Figure 5. Movement of bead-induced AChR clusters. One day after cluster formation induced by 5-µm beads, the clusters were followed for an additional 40 h. (A) Superimposed time-lapse image of the positions of four AChR clusters. (B–D) Corresponding phase–contrast images to show bead positions. Arrows in B point to two reference markers which showed little displacement during the 40-h period.

View larger version (38K): [in this window] [in a new window]   Figure 6. Trajectories of bead/AChR movement. (A and B) Four clusters and their associated beads were followed during a 24-h period. This image was taken at the end of the recording. The trajectories of these four beads are shown in C with the beginning and end points indicated. (D) Except for slow diffusional drift, the bead movement was abolished after Ltn A (1 µM) treatment. (E) MSD plotted against time. The linear slope of Ltn A data provides an estimate of the diffusion coefficient of the bead/AChR complex in the plane of the membrane. The solid curve is a regression fit of the control data using the equation described. From this, an actin-driven bead/cluster movement velocity of 0.1 nm/s is calculated. The R coefficient of the regression is 0.9985.

To quantify the bead movement, mean square displacement (MSD) was calculated at each time point according to the formula (Lee et al. 1991 ; Dai and Peng 1996 ).

where (x_n+i, y_n+i) is the position of the bead at time nt_o (t_o = interval between measurements) after starting at position (x_i, y_i). N = total number of positions recorded. n ranges from 1 to N - 1. Example MSD values in control and Ltn A–treated cultures are plotted in Fig 6 E. MSD plots from Ltn A–treated cells were largely linear, consistent with the notion that the small amount of bead movement is diffusion-limited. By fitting the MSD plots from Ltn A–treated cells with the following formula, the diffusion coefficient of bead movement was calculated.

where D is the diffusion coefficient and t is the elapsed time interval. A mean diffusion coefficient of 3 x 10^-14 cm²/s was obtained. This value was used to estimate the mean velocity of actin-driven bead movement from plots such as the one shown in Fig 6 E by fitting the MSD data with the following formula:

where v is the mean speed of actin-driven movement. From this calculation, a mean speed of 0.12 nm/s was obtained. As shown in Fig 6 E (solid line), this calculation gave very good fit to the data (R coefficient = 0.99). This value is consistent with those obtained from the trajectories shown in Fig 6 C.

These data show that AChR clusters with their associated beads undergo actin-driven movement in the plane of the membrane. As receptors within the cluster are connected with the bead via an extracellular matrix linkage and are linked with other cytoskeletal proteins such as the dystrophin complex, as we have shown previously (Peng and Chen 1992 ; Rochlin et al. 1989 ), the AChR cluster movement actually involves an entire transmembrane protein complex. In contrast to bead-induced AChR clusters, there is no evidence that preexistent hot spots, which are not associated with dynamic actin assembly, undergo movement (our unpublished observation).

The Effect of Ltn A and Jasplakinolide on AChR and PY Clustering
The colocalization of F-actin with AChR clusters also suggests a role of actin polymerization in their formation. This premise was tested by interfering with F-actin assembly with Ltn A. Muscle cultures were pretreated with Ltn A for 30 min and then presented with beads or agrin in the continued presence of this toxin. Ltn A, at a concentration as low as 1 µM, reduced F-actin assembly induced by beads (Fig 7A and Fig B). AChR clustering induced by beads was also reduced as shown by a decrease in the intensity of R-BTX labeling associated with beads (Fig 7 C). At higher Ltn A concentrations, cluster formation was abolished in a dose-dependent manner with half-inhibitory concentration for complete blockade at 20 µM (Fig 8, A–D and I). An example of this inhibition is shown in Fig 8A–D. At these concentrations, cytochalasin D had little effect on bead-induced AChR clustering (Fig 8 I).

View larger version (65K): [in this window] [in a new window]   Figure 7. The effect of Ltn A on AChR clustering. (A and B) Rh-phalloidin labeling after jasplakinolide masking. Ltn A at 1 µM diminished bead-induced actin polymerization. (C) Change in fluorescence intensity of bead-induced AChR clusters as shown by OG-BTX labeling resulting from Ltn A treatment.

View larger version (87K): [in this window] [in a new window]   Figure 8. Inhibition of AChR clustering by Ltn A. (A–D) Effect on bead-induced cluster formation. (A and B) In the untreated culture, clusters were induced by the majority of beads. Asterisks in B indicate cluster-positive beads. At the same time, preexistent AChR hot spots were dispersed as shown by the lack of non-bead-associated clusters. (C and D) Ltn A at 40 µM completely abolished the effect of beads in inducing new clusters, whereas preexisting hot spots were retained (C, arrow). (E–H) Effect on agrin-induced cluster formation. Control culture exhibited AChR hot spots (E) that generally were much larger than agrin-induced punctate clusters (F). Ltn A blocked the effect of agrin in a dose-dependent manner (G and H). Hot spots (arrows) were retained in the presence of Ltn A. (I) Quantification of the Ltn A effect. Cytochalasin D at comparable concentrations was ineffective in inhibiting cluster formation (only bead data shown). (J) Reversal of the Ltn A effect. While Ltn A inhibited both bead and agrin-induced AChR clustering (middle bars), cells recovered from this blockage and responded to these stimuli to the same level as control cells following its removal (right bars).

In addition to inducing new AChR clusters, HB-GAM beads invariably cause the dispersal of preexisting hot spots (Dai and Peng 1998 ). This effect is similar to nerve-induced dispersal of hot spots (Moody-Corbett and Cohen 1982b ; Kidokoro and Brass 1985 ). In the presence of Ltn A, the dispersal of hot spots induced by beads was also inhibited (Fig 8 C, arrow). Thus, Ltn A, for the duration of these experiments that last for 24 h, does not affect the stability of preexisting AChR clusters.

Ltn A also inhibited agrin-induced cluster formation in a dose-dependent manner (Fig 8, E–I). Hot spots, evidenced by their larger size as compared with the smaller agrin-induced clusters, also underwent dispersal in response to agrin treatment. Similar to the bead situation, they were also retained in agrin-treated cultures in the presence of Ltn A (Fig 8, G–H, arrows). Thus, F-actin assembly is also a necessary step involved in agrin-induced AChR clustering. Due to the fact that Ltn A inhibited neurite outgrowth, we were unable to assess the effect of Ltn A on NMJ formation in vitro.

To assess whether Ltn A's inhibition on clustering was due to deleterious effects on cell viability, the reversibility of this phenomenon was studied. Cells were treated with 40 µM of this toxin for 24 h, returned to normal medium, and then stimulated with beads or agrin. As shown in Fig 8 J, near complete recovery of AChR clustering was observed. Thus, the viability of the cells was not compromised by Ltn A treatment for the duration of these experiments.

In addition to Ltn A, the effect of jasplakinolide on AChR cluster formation was also studied. Although at low concentrations and for short durations this F-actin binding toxin did not inhibit AChR clustering as described above, prolonged treatment at higher concentrations did inhibit cluster formation as shown in Fig 9. Both bead- and agrin-induced clustering processes were inhibited in a dose-dependent manner by jasplakinolide (Fig 9 G). Clusters that still formed in the presence of high concentrations of jasplakinolide (50 µM) were much smaller than control clusters (Fig 9E and Fig F). Despite its effect on new cluster formation, hot spots remained stable in the presence of jasplakinolide (Fig 9 C).

View larger version (46K): [in this window] [in a new window]   Figure 9. Inhibition of AChR clustering by jasplakinolide. (A–D) Agrin-induced cluster formation was inhibited by jasplakinolide, but the hot spot was retained. (E and F) Bead-induced clustering was also inhibited. Clusters that formed in the presence of jasplakinolide (50 µM) were much smaller than control clusters. (G) Quantification of the inhibitory effect.

As tyrosine kinase activation is pivotal to AChR clustering, we sought to understand the relationship between actin polymerization and PY accumulation induced by beads. Consistent with our previous finding (Baker and Peng 1993 ), PY was accumulated at bead–muscle contacts (Fig 10, A–D). Similar to its effect on AChR clustering, Ltn A also blocked bead-induced PY accumulation (Fig 10, E–G). However, PY accumulation was unchanged at hot spots by Ltn A treatment (Fig 10, E–F, arrows). These data suggest that the accumulation of tyrosine-phosphorylated proteins at sites of postsynaptic stimulation requires F-actin assembly. The persistence of PY at hot spots is consistent with the relatively stable F-actin cytoskeleton at these specializations implicated by the results described above.

View larger version (91K): [in this window] [in a new window]   Figure 10. Disruption of PY accumulation by Ltn A. (A–D) Two examples of PY accumulation at HB-GAM bead-induced AChR clusters. (E–G) Ltn A (50 µM) treatment abolished AChR clustering (F) and PY accumulation (E) at bead–muscle contacts (G, asterisks), but left both AChRs and PY intact at preexisting hot spots (arrows).

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

In this work, we have documented the roles of actin polymerization induced by two NMJ stimuli with three main findings. (i) By masking preexisting F-actin structures with jasplakinolide, the new F-actin cytoskeleton induced by AChR clustering stimuli was clearly visualized. (ii) Actin polymerization at sites of AChR clustering is highly dynamic and generates enough force to propel the entire cluster complex to move along the membrane. (iii) F-actin assembly is necessary for the formation of AChR and PY clusters. These results indicate that actin polymerization triggered by synaptogenic signals is a pivotal event in AChR clustering and motility.

Relationship between F-Actin and AChRs
Our results indicate that actin polymerization can take place in two distinct configurations in response to synaptogenic signals. Responding to spatially discrete stimuli such as beads, muscle cells assemble F-actin locally and the resultant cytoskeleton conforms to the AChR cluster. The fact that we did not see a one-to-one relationship between F-actin and bead-induced AChR cluster can be explained by the fact that the former is a filamentous polymer whereas the latter is a globular protein. Thus, a single F-actin filament may accommodate multiple AChRs. On the other hand, bath application of agrin, which induces diffuse AChR clustering, results in a more global F-actin assembly that encompasses the region of AChR clusters but only occasionally colocalizes with them. On this point, we would like to offer two possible explanations. First, the newly polymerized F-actin at sites of AChR clusters may be variable and not extensive enough to be detected reliably with the method used. Second, AChR clusters induced by agrin may form on preexisting, localized F-actin cytoskeletal scaffolds and relatively low amounts of new actin polymerization is induced by agrin treatment at these sites. The latter hypothesis is being tested in our lab.

Previous studies with FRAP (fluorescence recovery after photobleaching) have shown that individual AChRs undergo lateral movement with a diffusion coefficient of 10^-10–10^-9 cm²/s, but clustered receptors are essentially immobile within the time span of the experiment lasting seconds to minutes (Kidokoro and Brass 1985 ; Axelrod et al. 1976 ; Peng et al. 1989 ). These results led to the diffusion trap hypothesis to account for AChR clustering (Poo 1985 ; Edwards and Frisch 1976 ), which depicts that freely moving AChRs become immobilized by a trapping mechanism locally set up as a result of innervation. It is generally believed that the postsynaptic cytoskeleton provides such a trap. Our results have now shown that actin polymerization is at least part of the mechanism of AChR immobilization.

AChRs may interact with actin filaments via rapsyn, a protein that associates with the cytoplasmic domain of AChR subunits (Sanes 1997 ; Porter and Froehner 1983 ; Maimone and Merlie 1993 ; Ramarao and Cohen 1998 ) and may bind directly to F-actin (Walker et al. 1984 ). A recent study has shown that AChR clustering in skeletal muscle is abolished in rapsyn-null mice (Gautam et al. 1995 ). When expressed in nonmuscle cells, either with or without AChR subunits, rapsyn forms clusters on its own independent of external stimuli (Froehner et al. 1990 ; Phillips et al. 1991 ; Scotland et al. 1993 ). Furthermore, these nonmuscle rapsyn clusters, like the postsynaptic AChR clusters, are associated with PY (Dai et al. 1996 ; Qu et al. 1996 ). Thus, rapsyn is capable of organizing membrane domains on its own, perhaps through its interaction with the cortical actin cytoskeleton. Local F-actin assembly during synaptogenesis may recruit rapsyn and its associated AChR to cluster (Burden 1985 ; Wallace 1989 ; Peng and Froehner 1985 ). Recent study has also shown that rapsyn can interact with MuSK through a putative transmembrane linker that interacts with rapsyn intracellularly and with MuSK's ectodomain extracellularly (Apel et al. 1997 ). It is thought that AChRs become clustered as a consequence of MuSK clustering. Thus, MuSK cluster could serve as a scaffold for AChR clustering. The fact that AChR clustering induced by agrin which acts through MuSK is abolished after Ltn A treatment suggests that F-actin assembly may also underlie the formation of the MuSK scaffold.

Recent studies have shown that cortical F-actin meshwork assembly induced by RTK activation is mediated by small GTPases Rac and Cdc42 (Ridley and Hall 1992 ; Machesky and Hall 1996 ; Ridley et al. 1992 ). The requirement of Rac and Cdc42 in agrin-induced AChR cluster formation in myotubes has recently been reported (Weston et al. 2000 ). Thus, this finding also implicates the role of actin polymerization in agrin-induced AChR clustering. Although our results have shown a diffuse F-actin assembly as a result of bath agrin application, local deposition of agrin during motor innervation (Cohen et al. 1994 ) would presumably result in localized actin polymerization similar to that induced by beads.

Although cytochalasins are widely used as inhibitors of F-actin assembly, they are ineffective in blocking AChR clustering, as reported here. Their effect on preexisting AChR hot spots is inconsistent. Hot spots in chick myotubes are disrupted by cytochalasins (Connolly 1984 ), but those in cultured Xenopus muscle cells are not (Moody-Corbett and Cohen 1982a ). The mechanism underlying cytochalasins' inhibition of actin polymerization is different from that of Ltn A. The latter binds to G-actin and sequesters it from polymerization into F-actin (Spector et al. 1983 , Spector et al. 1989 ; Coue et al. 1987 ), whereas the former binds to the barbed end of actin filament but does not inhibit monomer addition to the pointed end nor does it completely block addition to the barbed end of the elongating filament (Bonder and Mooseker 1986 ). Thus, Ltn A is a much more potent inhibitor of actin polymerization (Coue et al. 1987 ; Spector et al. 1989 ). The action of jasplakinolide in inhibiting cluster formation is different from that of Ltn A. The latter blocks actin polymerization, whereas the former actually induces actin polymerization and/or stabilizes preexisting actin filaments due to its binding to F-actin (Bubb et al. 1994 ). Thus, it may promote F-actin assembly in the entire muscle cell, thereby leading to a decrease in AChR mobility as a result of its interaction with the cytoskeleton. This may cause a depletion of the mobile AChR pool for cluster formation. Alternatively, it may bind to and stabilize incompletely assembled actin filaments induced by synaptogenic signals to interfere with the dynamic cytoskeletal assembly necessary for AChR cluster formation. The diminution in the size of AChR clusters that still form in the presence of jasplakinolide is consistent with the latter notion.

Actin-driven Cluster Movement
The novel actin-driven AChR cluster movement appears qualitatively similar to the "inductopodia" induced by polycationic beads on Aplysia growth cones (Forscher et al. 1992 ). In that case, F-actin assembly induced by submicron beads produces sufficient force to propel them to move on the growth cone surface. Growth cones also exhibit another kind of actin-based protrusive structure termed intrapodium, generated by a burst of actin polymerization resulting from the removal of cytoskeletal disrupting agents such as cytochalasin and nocodazole (Rochlin et al. 1999 ). Actin-driven movements have also been well documented on intracellular pathogens such as Listeria and Vaccinia and on cytoplasmic Arp2/3 spots (Theriot 1995 ; Cudmore et al. 1995 ; Schafer et al. 1998 ).

In addition to AChRs, the cargo moved by polymerizing actin most likely includes other AChR-associated proteins. Our previous studies have shown that 1-d bead-induced AChR clusters similar to the ones studied here already have a basement membrane in the cleft space between the bead and the cell (Peng and Chen 1992 ). In addition, these clusters are associated with cytoskeletal and signaling proteins including rapsyn, dystrophin complex (dystrophin, syntrophin, and dystroglycan), and focal adhesion kinase (Peng and Froehner 1985 ; Chen et al. 1990 ; Baker et al. 1994 ; Peng et al. 1998 ). Furthermore, the AChR-rich membrane at bead–muscle contacts is already deeply invaginated at this time (Peng and Chen 1992 ). Thus, the bead-associated cluster movement involves an entire transmembrane protein complex as well as complicated membrane topography.

Quantitatively, the velocity of the AChR cluster movement is three orders of magnitude slower than other actin-driven movements described above (Theriot et al. 1992 ; Schafer et al. 1998 ). A reason for this difference could be that the AChR cluster complex is tethered to a global cortical cytoskeleton and the connections have to be modified as the cluster moves along the membrane. The fact that these clusters move at all is indicative of the considerable propulsive force generated by actin polymerization induced by synaptogenic stimuli.

The physiological significance of this receptor cluster movement is not known. It could serve as a mechanism for coalescing small clusters into large ones during NMJ formation. Although the movement reported here is nondirectional, it is conceivable that during innervation, AChR patches can move in a preferred direction as a result of the directional neurite extension on the muscle cell. A directional cluster movement may serve to remodel or to enlarge the postsynaptic membrane. The movement of large transmembrane protein complex is also exemplified by the recent demonstration of focal adhesion translocation in stationary fibroblasts (Smilenov et al. 1999 ).

Dynamics of F-actin Assembly
From both jasplakinolide masking and cortactin–EGFP localization results, the dynamic state of the F-actin cytoskeleton at new versus existing AChR clusters is contrasted. The use of cortactin to mark sites of new actin assembly is supported by findings that show its localization at leading edge as described above. In addition, cortactin also binds to dynamic Arp2/3-positive actin spots in fibroblasts (Kaksonen, M., H.B. Peng, and H. Rauvala, manuscript in preparation). It does not bind to stress fibers or myofibrils (Wu and Parsons 1993 ; Peng et al. 1997 ). The cortactin–EGFP data presented here are consistent with our previous immunofluorescence study, which showed that endogenous cortactin is localized at newly formed AChR clusters induced by beads (Peng et al. 1997 ). As shown here, newly induced AChR clusters are associated with new actin assembly, which remains dynamic as shown by its association with cortactin. Preexisting hot spots have little newly polymerized F-actin and cortactin and thus the cytoskeleton at these sites seems stable. These results are consistent with the differential effects of Ltn A on new versus preexisting AChR clusters. Ltn A inhibits the formation of new clusters, but it has little effect on hot spots.

In contrast to preexisting AChR clusters in muscle cells, NMDA and AMPA receptor clusters located on cultured hippocampal neurons are dispersed after Ltn A treatment (Allison et al. 1998 ). In fact, Ltn A disrupts the F-actin cytoskeleton within dendritic spines on which these glutamate receptors are clustered. This suggests that the spine actin cytoskeleton is maintained in a dynamic state, probably similar to that at newly formed AChR clusters in cultured muscle cells. Consistent with this notion is the finding that cortactin is also enriched in the postsynaptic density associated with these central receptor clusters (Boeckers et al. 1999 ; Naisbitt et al. 1999 ). Thus, the actin-driven motility of the postsynaptic apparatus described here may also be applicable to central synapses, where it may play a role in synaptic plasticity.

The Actin Cytoskeleton As a Scaffold for Postsynaptic Signaling
In addition to cytoskeletal proteins, the F-actin specialization may also provide a mechanism for the anchorage of signaling molecules as evidenced by the necessity of its assembly in PY accumulation. The identity of tyrosine-phosphorylated proteins at the AChR cluster is unknown. Several studies have shown that the ß-subunit of AChR is tyrosine-phosphorylated upon MuSK activation (Hopf and Hoch 1998 ; Apel et al. 1997 ; Glass et al. 1997 ). However, recent data have shown that proteins other than the receptor are responsible for the early PY accumulation at sites of AChR clustering, and ß-subunit tyrosine phosphorylation is not necessary for cluster formation (Baker and Peng 1993 ; Meyer and Wallace 1998 ). In addition to MuSK, several other kinases, such as focal adhesion kinase and Src, are known to be either concentrated or activated by synaptogenic signals (Fuhrer and Hall 1996 ; Baker et al. 1994 ). AChR clusters are also associated with other signaling and structural proteins that are tyrosine kinase substrates such as Grb 2, dystrobrevin, and cortactin (Colledge and Froehner 1997 ; Peng et al. 1997 ; Grady et al. 2000 ). These proteins are candidates for the observed PY accumulation at AChR clusters. Besides AChR cluster formation, our results suggest an additional role of the F-actin assembly in hot spot dispersal, since Ltn A treatment abolishes the dispersal of preexisting hot spots (Fig 8). Our recent study has implicated the role of tyrosine phosphatases in preexisting AChR cluster dispersal (Dai and Peng 1998 ). Thus, the actin cytoskeleton may also play a role in phosphatase signaling. In sum, actin polymerization induced by synaptogenic signals may lead to the assembly of a scaffold for both structural and signaling molecules necessary for the formation of the postsynaptic apparatus.

	Footnotes

¹ Abbreviations used in this paper: AChR, acetylcholine receptor; F-actin, filamentous actin; G-actin, globular actin; GFP, green fluorescent protein; HB-GAM, heparin-binding growth-associated molecule; Ltn A, latrunculin A; MSD, mean-square displacement; MuSK, muscle-specific kinase; NMJ, neuromuscular junction; OG-BTX, Oregon green–conjugated -bungarotoxin; PY, phosphotyrosine; Rh-phalloidin, rhodamine-conjugated phalloidin; R-BTX, rhodamine-conjugated -bungarotoxin; RTK, receptor-tyrosine kinase.

	Acknowledgements

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

We thank Drs. Tom Parsons, Heikki Rauvala, and Rick Rotundo, as well as Mr. Marko Kaksonen, for providing reagents, and Dr. R. Madhavan for comments on the manuscript.

This work was supported by National Institutes of Health grant NS-23583 and the Muscular Dystrophy Association.

Submitted: 19 April 2000
Revised: 27 June 2000
Accepted: 18 July 2000

	References

Top Abstract Introduction Materials and Methods Results Discussion Acknowledgements References

1. Allison, D.W., Gelfand, V.I., Spector, I., and Craig, A.M. 1998. Role of actin in anchoring postsynaptic receptors in cultured hippocampal neurons: differential attachment of NMDA versus AMPA receptors. J. Neurosci. 18:2423-2436[Abstract/Free Full Text].

2. Apel, E.D., Glass, D.J., Moscoso, L.M., Yancopoulos, G.D., and Sanes, J.R. 1997. Rapsyn is required for MuSK signaling and recruits synaptic components to a MuSK-containing scaffold. Neuron. 18:623-635[Medline].

3. Axelrod, D., Ravdin, P., Koppel, D.E., Schlessinger, J., Webb, W.W., Elson, E.L., and Podleski, T.R. 1976. Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proc. Natl. Acad. Sci. USA. 73:4954-4958.

4. Baker, L.P., Daggett, D.F., and Peng, H.B. 1994. Concentration of pp125 focal adhesion kinase (FAK) at the myotendinous junction. J. Cell Sci. 107:1485-1497[Abstract].

5. Baker, L.P., and Peng, H.B. 1993. Tyrosine phosphorylation and acetylcholine receptor cluster formation in cultured Xenopus muscle cells. J. Cell Biol. 120:185-195[Abstract/Free Full Text].

6. Bloch, R.J. 1986. Actin at receptor-rich domains of isolated acetylcholine receptor clusters. J. Cell Biol. 102:1447-1458[Abstract/Free Full Text].

7. Boeckers, T.M., Kreutz, M.R., Winter, C., Zuschratter, W., Smalla, K.H., Sanmarti-Vila, L., Wex, H., Langnaese, K., Bockmann, J., Garner, C.C., and Gundelfinger, E.D. 1999. Proline-rich synapse-associated protein-1/Cortactin binding protein 1 (ProSAP1/CortBP1) is a PDZ-domain protein highly enriched in the postsynaptic density. J. Neurosci. 19:6506-6518[Abstract/Free Full Text].

8. Bonder, E.M., and Mooseker, M.S. 1986. Cytochalasin B slows but does not prevent monomer addition at the barbed end of the actin filament. J. Cell Biol. 102:282-288[Abstract/Free Full Text].

9. Bowe, M.A., and Fallon, J.R. 1995. The role of agrin in synapse formation. Annu. Rev. Neurosci. 18:443-462[Medline].

10. Bubb, M.R., Senderowicz, A.M., Sausville, E.A., Duncan, K.L., and Korn, E.D. 1994. Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. J. Biol. Chem. 269:14869-14871[Abstract/Free Full Text].

11. Burden, S.J. 1985. The subsynaptic 43kDa protein is concentrated at developing nerve-muscle synapses in vitro. Proc. Natl. Acad. Sci. USA. 82:8270-8273[Abstract/Free Full Text].

12. Chen, Q., Sealock, R., and Peng, H.B. 1990. A protein homologous to the Torpedo postsynaptic 58K protein is present at the myotendinous junction. J. Cell Biol. 110:2061-2071[Abstract/Free Full Text].

13. Cohen, M.W., Moody-Corbett, F., and Godfrey, E.W. 1994. Neurite deposition of agrin on culture substrate: implication for nerve-muscle synaptogenesis. J. Neurosci. 14:3293-3303[Abstract].

14. Colledge, M., and Froehner, S.C. 1997. Tyrosine phosphorylation of nicotinic acetylcholine receptor mediates Grb2 binding. J. Neurosci. 17:5038-5045[Abstract/Free Full Text].

15. Condeelis, J. 1993. Life at the leading edge: the formation of cell protrusions. Annu. Rev. Cell Biol. 9:411-444.

16. Connolly, J.A. 1984. Role of the cytoskeleton in the formation, stabilization and removal of acetylcholine receptor clusters in cultured muscle cells. J. Cell Biol. 99:148-154[Abstract/Free Full Text].

17. Coue, M., Brenner, S.L., Spector, I., and Korn, E.D. 1987. Inhibition of actin polymerization by latrunculin A. FEBS (Fed. Eur. Biochem. Soc.) Lett. 213:316-318.

18. Crews, P., Manes, L.V., and Boehler, M. 1986. Jasplakinolide, a cyclodepsipeptide from the marine sponge, Jaspis sp. Tetrahedron Lett. 27:C691-C700.

19. Cudmore, S., Cossart, P., Griffiths, G., and Way, M. 1995. Actin-based motility of vaccinia virus. Nature. 378:636-638[Medline].

20. Daggett, D.F., Cohen, M.W., Stone, D., Nikolics, K., Rauvala, H., and Peng, H.B. 1996a. The role of an agrin-growth factor interaction in ACh receptor clustering. Mol. Cell. Neurosci. 8:272-285[Medline].

21. Daggett, D.F., Stone, D., Peng, H.B., and Nikolics, K. 1996b. Full-length agrin isoform activities and binding site distributions on cultured Xenopus muscle cells. Mol. Cell. Neurosci. 7:75-88[Medline].

22. Dai, Z., and Peng, H.B. 1996. Dynamics of synaptic vesicles in cultured spinal cord neurons in relationship to synaptogenesis. Mol. Cell. Neurosci. 7:443-452[Medline].

23. Dai, Z., and Peng, H.B. 1998. A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation. J. Cell Biol. 141:1613-1624[Abstract/Free Full Text].

24. Dai, Z., Scotland, P.B., Froehner, S.C., and Peng, H.B. 1996. Association of phosphotyrosine with rapsyn expressed in Xenopus embryonic cells. Neuroreport. 7:657-661[Medline].

25. Edwards, C., and Frisch, H.L. 1976. A model for the localization of acetylcholine receptors at the muscle endplate. J. Neurobiol. 7:377-381[Medline].

26. Forscher, P., Lin, C.H., and Thompson, C. 1992. Novel form of growth cone motility involving site-directed actin filament assembly. Nature. 357:515-518[Medline].

27. Froehner, S.C., Luetje, C.W., Scotland, P.B., and Patrick, J. 1990. The postsynaptic 43K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes. Neuron. 5:403-410[Medline].

28. Fuhrer, C., and Hall, Z.W. 1996. Functional interaction of Src family kinases with the acetylcholine receptor in C2 myotubes. J. Biol. Chem. 271:32474-32481[Abstract/Free Full Text].

29. Ganju, P., Walls, E., Brennan, J., and Reith, A.D. 1995. Cloning and developmental expression of Nsk2, a novel receptor tyrosine kinase implicated in skeletal myogenesis. Oncogene. 11:281-290[Medline].

30. Gautam, M., Noakes, P.G., Mudd, J., Nichol, M., Chu, G.C., Sanes, J.R., and Merlie, J.P. 1995. Failure of postsynaptic specialization to develop at neuromuscular junctions of rapsyn-deficient mice. Nature. 377:232-236[Medline].

31. Gautam, M., Noakes, P.G., Moscoso, L., Rupp, F., Scheller, R.H., Merlie, J.P., and Sanes, J.R. 1996. Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell. 85:525-535[Medline].

32. Glass, D.J., Apel, E.D., Shah, S., Bowen, D.C., DeChiara, T.M., Stitt, T.N., Sanes, J.R., and Yancopoulos, G.D. 1997. Kinase domain of the muscle-specific receptor tyrosine kinase (MuSK) is sufficient for phosphorylation but not clustering of acetylcholine receptors: required role for the MuSK ectodomain? Proc. Natl. Acad. Sci. USA. 94:8848-8853[Abstract/Free Full Text].

33. Glass, D.J., Bowen, D.C., Stitt, T.N., Radziejewski, C., Bruno, J., Ryan, T.E., Gies, D.R., Shah, S., Mattsson, K., and Burden, S.J. et al. 1996. Agrin acts via a MuSK receptor complex. Cell. 85:513-523[Medline].

34. Grady, R.M., Zhou, H., Cunningham, J.M., Henry, M.D., Campbell, K.P., and Sanes, J.R. 2000. Maturation and maintenance of the neuromuscular synapse: genetic evidence for roles of the dystrophin--glycoprotein complex. Neuron. 25:279-293[Medline].

35. Hopf, C., and Hoch, W. 1998. Dimerization of the muscle-specific kinase induces tyrosine phosphorylation of acetylcholine receptors and their aggregation on the surface of myotubes. J. Biol. Chem. 273:6467-6473[Abstract/Free Full Text].

36. Kidokoro, Y., and Brass, B. 1985. Redistribution of acetylcholine receptors during neuromuscular junction formation in Xenopus cultures. J. Physiol. 80:212-220.

37. Lee, G.M., Ishihara, A., and Jacobson, K.A. 1991. Direct observation of Brownian motion of lipids in a membrane. Proc. Natl. Acad. Sci. USA. 88:6274-6278[Abstract/Free Full Text].

38. Littlefield, R., and Fowler, V.M. 1998. Defining actin filament length in striated muscle: rulers and caps or dynamic stability? Annu. Rev. Cell Dev. Biol. 14:487-525[Medline].

39. Machesky, L.M., and Hall, A. 1996. Rho: a connection between membrane receptor signalling and the cytoskeleton. Trends Cell Biol. 6:304-310[Medline].

40. Maimone, M.M., and Merlie, J.P. 1993. Interaction of the 43 kd postsynaptic protein with all subunits of the muscle nicotinic acetylcholine receptor. Neuron. 11:53-66[Medline].

41. Meyer, G., and Wallace, B.G. 1998. Recruitment of a nicotinic acetylcholine receptor mutant lacking cytoplasmic tyrosine residues in its beta subunit into agrin-induced aggregates. Mol. Cell. Neurosci. 11:324-333[Medline].

42. Moody-Corbett, F., and Cohen, M.W. 1982a. Increased adhesiveness at sites of high acetylcholine receptor density on embryonic amphibian muscle cells cultured without nerve. J. Embryol. Exp. Morphol. 72:53-69[Medline].

43. Moody-Corbett, F., and Cohen, M.W. 1982b. Influence of nerve on the formation and survival of acetylcholine receptor and cholinesterase patches on embryonic Xenopus muscle cells in culture. J. Neurosci. 2:633-646[Abstract].

44. Morton, W.M., Ayscough, K.R., and McLaughlin, P.J. 2000. Latrunculin alters the actin-monomer subunit interface to prevent polymerization. Nat. Cell Biol. 2:376-378[Medline].

45. Naisbitt, S., Kim, E., Tu, J.C., Xiao, B., Sala, C., Valtschanoff, J., Weinberg, R.J., Worley, P.F., and Sheng, M. 1999. Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin. Neuron. 23:569-582[Medline].

46. Ojaniemi, M., and Vuori, K. 1997. Epidermal growth factor modulates tyrosine phosphorylation of p130Cas. Involvement of phosphatidylinositol 3'-kinase and actin cytoskeleton. J. Biol. Chem. 272:25993-25998[Abstract/Free Full Text].

47. Peng, H.B., and Phelan, K.A. 1984. Early cytoplasmic specialization at the presumptive acetylcholine receptor cluster: a meshwork of thin filaments. J. Cell Biol. 99:344-349[Abstract/Free Full Text].

48. Peng, H.B., and Froehner, S.C. 1985. Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters. J. Cell Biol. 100:1698-1705[Abstract/Free Full Text].

49. Peng, H.B., and Chen, Q. 1992. Induction of dystrophin localization in cultured Xenopus muscle cells by latex beads. J. Cell Sci. 103:551-563[Abstract].

50. Peng, H.B., Zhao, D.-Y., Xie, M.-Z., Shen, Z., and Jacobson, K. 1989. The role of lateral migration in the formation of acetylcholine receptor clusters induced