JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 13 May 2002. doi:10.1083/jcb1574iti4
This Article
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dove, A. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Dove, A. W.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2002/5/545-a $5.00
The Journal of Cell Biology, Volume 157, Number 4, May 13, 2002 545-a-545

In This Issue

 Arresting development
MISS depeletion (bottom) causes defects in meiosis II spindles.

In meiosis II, vertebrate oocytes undergo a cell cycle arrest that is maintained until fertilization, but it has been difficult to identify the factors responsible for this process. By screening a novel cDNA library, Lefebvre et al., page 603, have discovered and characterized a protein that specifically regulates meiosis II spindle integrity during arrest. The work helps to explain how the cell distinguishes between meiosis I and II, and the new library should be useful in discovering additional genes involved in early development.

Immature oocytes do not initiate an arrest until they reach meiosis II, but the kinases known to trigger this process are expressed through both meiotic divisions, so other factors must determine how and when the arrest occurs. The authors generated a cDNA library from mouse immature oocytes, representing transcripts that are specifically relevant to meiotic maturation. Two-hybrid screening identified a MAP kinase-interacting and spindle-stabilizing protein (MISS). MISS is unstable in meiosis I, but becomes stable in meiosis II, when it localizes in dots on the spindles. Interfering with endogenous MISS RNA causes severe spindle defects only in meiosis II. Its specific stabilization during meiosis II suggests that MISS provides a link between MAP kinase activity and meiotic arrest. {blacksquare}



Alan W. Dove

alanwdove{at}earthlink.net


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Meiotic spindle stability depends on MAPK-interacting and spindle-stabilizing protein (MISS), a new MAPK substrate
Christophe Lefebvre, M. Emilie Terret, Alexandre Djiane, Pascale Rassinier, Bernard Maro, and Marie-Hélène Verlhac
J. Cell Biol. 2002 157: 603-613. [Abstract] [Full Text] [PDF]




This Article
Right arrow PDF (Full Text)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dove, A. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Dove, A. W.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents