Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article PDF (Full Text) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dove, A. W. Search for Related Content PubMed Articles by Dove, A. W. Related Collections Related Article Social Bookmarking                      What's this?

PF2 acts as a molecular scaffold in flagella.

The movements of cilia and flagella are driven by axonemal dynein ATPases, whose activity must be coordinated in space and time. On page 47, Rupp and Porter provide the first molecular characterization of a component of the dynein regulatory complex (DRC), a crucial but poorly understood regulator of flagellar movement. Besides suggesting a model for DRC assembly, the work identifies a possible connection between motility regulation and a theoretical mechanism of cell growth arrest.

Using insertional mutagenesis in Chlamydomonas, the authors identified a new mutation in the PF2 locus that causes motility defects. Further analysis showed that PF2 encodes subunit 4 of the DRC. The PF2 protein is uniformly distributed along the length of the axoneme and also associates with the basal body region, and its predicted structure has several coiled-coil domains that could mediate protein–protein interactions. PF2 mutants fail to assemble five of the seven known DRC subunits.

The results suggest that PF2 acts as a molecular scaffold, stabilizing the DRC by interacting with other components of the complex. Close homologues of PF2 occur in a wide range of cell types, and include a trypanosome gene product required for directional motility, and mouse and human gene products enriched in growth-arrested cells. One exciting possibility is that primary cilia containing PF2 homologues may transmit a signal to the cell to initiate growth arrest. As a first step in confirming this idea, the localization of PF2-related products must be determined in cells lacking flagella.

Alan W. Dove

alanwdove{at}earthlink.net

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest–specific gene product

Gerald Rupp and Mary E. Porter
J. Cell Biol. 2003 162: 47-57. [Abstract] [Full Text] [PDF]

This Article PDF (Full Text) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dove, A. W. Search for Related Content PubMed Articles by Dove, A. W. Related Collections Related Article Social Bookmarking                      What's this?