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Correspondence to Jan M.A. van Deursen: vandeursen.jan{at}mayo.edu
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Recently, we have reported the generation of a series of mice in which the expression of BubR1 is reduced in a graded fashion from normal levels to zero by the use of wild-type, knockout, and hypomorphic alleles (Baker et al., 2004). Of this series, mice with BubR1 levels as low as 10% of normal (BubR1H/H mice) are born alive and develop into adult mice. Remarkably, despite having severe aneuploidy, these animals do not have an escalated spontaneous tumor burden (Baker et al., 2004). Instead, these mice develop a variety of progeroid (resembling old age) features. In addition, mouse embryonic fibroblasts (MEFs) from BubR1H/H mice show profound premature cellular senescence. These observations, combined with the demonstration that BubR1 levels decline in ovary, testis, and spleen tissue as wild-type mice age, have suggested that this mitotic checkpoint protein is a key regulator of the normal aging process. However, whether aging is a general feature of spindle assembly checkpoint dysfunction remains unknown.
The mitotic checkpoint protein Bub3 is a seven-blade ß propeller that has substantial sequence similarity with the mRNA export factor protein Rae1; the two proteins have 34% identity and 52% similarity in humans (Larsen and Harrison, 2004). Like Bub3 (Kalitsis et al., 2000), Rae1 is essential for early mouse embryogenesis, but mice that are haploinsufficient for Bub3 or Rae1 are viable and have a normal appearance (Babu et al., 2003). These heterozygous mice show remarkably similar mitotic defects, including spindle assembly checkpoint impairment and chromosome missegregation (Babu et al., 2003). Mice that are haploinsufficient for both Bub3 and Rae1 are also viable but develop much more severe mitotic phenotypes than single haploinsufficient mice. In this study, we have investigated the long-term phenotypes of mice in which the mitotic checkpoint proteins Bub3 and Rae1 are disrupted individually or in combination. We find that phenotypes associated with aging appear early in double haploinsufficient mice but not in single haploinsufficient mice. We also find that single and double haploinsufficient mice are not predisposed to spontaneous tumor development despite the accumulation of substantial numbers of aneuploid cells.
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3 mo in the Bub3+/–/Rae1+/– group. This difference is mirrored by the reduction in tumor latency by 4 mo. Thus, mice in which the mitotic checkpoint genes Bub3 and Rae1 are disrupted individually or in combination are not predisposed to spontaneous tumorigenesis.
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Combined haploinsufficiency of Bub3 and Rae1 results in premature senescence
We next investigated whether combined haploinsufficiency of Bub3 and Rae1 triggers cell death or cellular senescence, which are two processes that have been linked to aging (Campisi, 2005; Lombard et al., 2005). We intercrossed Bub3+/– and Rae1+/– mice to produce Bub3+/–/Rae1+/–, Bub3+/–, Rae1+/–, and wild-type MEFs from 13.5-d-old fetuses. At passage 5 (P5), we assayed Bub3+/–/Rae1+/– and wild-type MEFs for cell death by TUNEL staining. We observed no difference in amounts of TUNEL-positive cells in Bub3+/–/Rae1+/– and wild-type MEFs cultures (Fig. 6 A), indicating that combined haploinsufficiency of Bub3 and Rae1 did not increase the rate of cell death. Combined annexin V–FITC and propidium iodide (PI) staining of P5 Bub3+/–/Rae1+/– and wild-type MEFs showed that neither apoptotic nor nonapoptotic cell death was significantly increased in Bub3+/–/Rae1+/– MEFs (Fig. 6 B) despite high rates of chromosome missegregation in mitosis. This is remarkable because high rates of cell death have been observed in studies in which the mitotic checkpoint proteins Mad2 and BubR1 were depleted by short inhibitory RNA (Kops et al., 2004; Meraldi et al., 2004; Michel et al., 2004). Thus, it seems that cell death only occurs when mitotic checkpoint proteins drop below a critical threshold level and that aberrant chromosome segregation itself is insufficient to trigger cell death.
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The early aging–associated phenotypes that develop in Bub3+/–/Rae1+/– mice resemble those detected in mice with low levels of BubR1 (Baker et al., 2004). However, BubR1H/H mice had a much earlier onset of aging phenotypes than Bub3+/–/Rae1+/– mice. Consistent with this, BubR1 hypomorphic MEF cultures contained significantly higher numbers of SA ß-galactosidase–positive cells than Bub3+/–/Rae1+/– MEF cultures (Fig. 6 E). In addition, BubR1H/H MEFs showed a more robust activation of the p53 and p16 pathways than Bub3+/–/Rae1+/– MEFs (Fig. 7 B). These data draw a clear correlation between the level of induction of senescence response genes and the rate of premature aging.
To further test this correlation, we prepared MEFs from Mad2 haploinsufficient mice and measured the degree of senescence at P3, P5, and P7. At each passage, the percentage of SA ß-galactosidase–positive Mad2+/– MEFs was similar to that of wild-type MEFs (Fig. S1 A, available at http://www.jcb.org/cgi/content/full/jcb.200507081/DC1). Furthermore, at each passage, p53, p21, p19, and p16 levels of Mad2+/– MEFs were comparable with those of wild-type MEFs (Fig. S1 B). Consistent with these findings, we observed no early aging–related phenotypes in the small cohort of Mad2+/– mice that we followed for a period of 19–27 mo (Fig. S1 C). Chromosome counts showed that 18% of Mad2+/– splenocytes were aneuploid when mice were 5 mo of age (Fig. S1 D). Thus, Mad2 haploinsufficiency does not promote cellular senescence in MEFs and does not seem to trigger aging-related phenotypes in mice, although aneuploidy is increased.
Spindle assembly checkpoint activity measurements
Our chromosome counts suggested that there is a rather weak link between checkpoint dysfunction and the early onset of senescence and aging. To further investigate this issue, we obtained two additional measures for checkpoint activity by methods involving live cell imaging. The first assay is a nocodazole challenge assay. MEFs carrying various mitotic checkpoint gene defects were first transduced with a retrovirus carrying a YFP-tagged H2B gene to allow visualization of chromosomes by fluorescent microscopy. We then challenged the MEFs with nocodazole and measured the duration of the ensuing checkpoint-dependent mitotic arrest. This duration of arrest in mitosis was defined as the interval between nuclear envelope breakdown (NEBD; onset of mitosis) and chromatin decondensation (exit from mitosis without cytokinesis). We used the time at which 50% of the cells had exited mitosis for comparison. Nocodazole-challenged wild-type MEFs typically remained arrested in prometaphase for 7.2 h (Fig. 8 A). However, single haploinsufficient MEFs of Bub3 and Rae1 showed a profound decrease in their ability to maintain this arrest, with 50% of the cells exiting at 3 h. Bub3+/–/Rae1+/– MEFs had an even further reduction in their ability to sustain arrest, with 50% of the cells exiting mitosis at 2.2 h. BubR1+/H and BubR1+/– MEFs, which express 60 and 30% of normal BubR1 protein, respectively (Baker et al., 2004), also had significant reductions in their ability to maintain a mitotic arrest, with 50% of the cells exiting at 5.3 and 3.8 h (Fig. 8 B). BubR1H/H MEFs (10% BubR1) had the most profound inability to maintain mitotic arrest, with 50% of the cells exiting at 1.1 h. It surprised us that the duration of arrest of BubR1H/H MEFs was substantially shorter than that of Bub3+/–/Rae1+/– MEFs, because BubR1H/H MEFs have less severe aneuploidy than Bub3+/–/Rae1+/– MEFs (Table II; also see Discussion). As an alternative measure for spindle checkpoint activity, we measured the accuracy of chromosome segregation during mitosis. In essence, we followed YFP-H2B–positive MEFs through an unchallenged mitosis by live cell imaging and determined the fraction of mitotic cells with chromosome segregation defects. We observed little or no misaligned chromosomes in wild-type, Bub3+/–, Rae1+/–, Bub3+/–/Rae1+/–, and BubR1H/H metaphases (Table III). However, a relatively large proportion of Bub3+/–/Rae1+/– and BubR1H/H anaphases had lagging chromosomes (Table III, Videos 1–3, and Fig. S4, available at http://www.jcb.org/cgi/content/full/jcb.200507081/DC1). The incidence of lagging chromosomes was also increased in Bub3+/– and Rae1+/– anaphases, but to a lesser extent. It should be noted that lagging chromosomes are not necessarily a sign of failed mitotic checkpoint signaling because they could result from merotelically attached kinetochores that do not activate the mitotic checkpoint (Cimini et al., 2001). Bub3+/–/Rae1+/– MEFs had not only a somewhat higher percentage of anaphases with lagging chromosomes than BubR1H/H MEFs but also a higher percentage of abnormal anaphases with more than one lagging chromosome (Table III and Video 3). This may explain why the spectrum of chromosome losses and gains is wider for Bub3+/–/Rae1+/– MEFs than for BubR1H/H MEFs (Table II). Collectively, the aforementioned data confirm that spindle assembly checkpoint dysfunction and chromosome missegregation do not correlate well with cellular senescence and aging.
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In the study of hypomorphic BubR1 mice (Baker et al., 2004), it became apparent that as these mice began to show signs of premature aging, they also started to accumulate aneuploid splenocytes. This led to the hypothesis that aneuploidy might be the primary lesion triggering cellular senescence and the process of aging. The use of Bub3 and Rae1 haploinsufficient mice allowed for rigorous testing of this hypothesis. At 5 mo, BubR1 hypomorphic mice have an aged appearance with detectable aneuploidy in 15% of their splenocytes (Baker et al., 2004). Age-matched single heterozygous Bub3 and Rae1 mice each have 9% aneuploid splenocytes but show no signs of early aging. One could argue that this may be the result of having aneuploidy that remains below a critical level. Splenocytes from 5-mo-old compound Bub3/Rae1 mice have 36% aneuploidy and a wide spectrum of chromosome losses and gains (Table I), but these animals show no overt features of aging at this age. If aneuploidy alone was a driving force of aging, these mice should have shown phenotypes of aging earlier than BubR1 hypomorphic mice. It is possible that the degree of aneuploidy in splenocytes is not representative of the degree present in tissues and cell types that develop early aging phenotypes. Although we cannot exclude this possibility with our current methods for measuring aneuploidy in vivo, the view that aneuploidy does not correlate with aging is further underscored by the finding that Bub3+/–/Rae1+/– MEFs are more aneuploid than BubR1 hypomorphic MEFs but exhibit lower levels of cellular senescence. In contrast, in mice with mitotic checkpoint gene defects, the rate of aging strongly correlates with the rate of cellular senescence. The induction of p53, p21, and p19 in both BubR1 hypomorphic and combined Bub3/Rae1 haploinsufficient cells but not Bub3 and Rae1 single haploinsufficient cells suggests that the lesion triggering cellular senescence and aging activates the p53 pathway. Like BubR1H/H MEFs (Baker et al., 2004), Bub3+/–/Rae1+/– MEFs have no detectable defects in DNA repair, suggesting that the lesion that triggers p53 activation is unlikely to be the accumulation of DNA damage or mutations. Various stress signals other than DNA damage are known to activate the p53 pathway (Ben-Porath and Weinberg, 2005; Harris and Levine, 2005), and future experiments will be necessary to determine whether any of these signals might be active in BubR1 hypomorphic and Bub3/Rae1 mutant mice.
Rae1, Bub3, and BubR1 have all been implicated in functions outside of the spindle assembly checkpoint. For example, Rae1 was originally discovered as a nucleocytoplasmic transport factor that regulates the export of mRNA from the nucleus (Brown et al., 1995; Kraemer and Blobel, 1997; Pritchard et al., 1999). How Rae1 regulates nuclear transport and whether Bub3 functions also in the transport machinery has not yet been established. We found that combined Bub3/Rae1 haploinsufficiency does not cause overt defects in global mRNA export or nuclear pore complex biogenesis (Fig. S2, available at http://www.jcb.org/cgi/content/full/jcb.200507081/DC1). However, our experiments by no means represent a comprehensive analysis of the transport machinery, and it is certainly possible that Bub3/Rae1 haploinsufficient cells have subtle trafficking defects. A recent study has suggested that Bub3 can interact with histone deacetylases and that, in this context, Bub3 might act to repress gene transcription in interphase (Yoon et al., 2004). BubR1 can also repress gene transcription in a heterologous DNA-binding context, although not in conjunction with histone deacetylases (Yoon et al., 2004). BubR1 also seems to mediate apoptosis of cells that exit mitosis without chromosome segregation (Shin et al., 2003), and, in yeast, the BubR1 homologue Mad3 and Bub3 have both been linked to the accumulation of gross chromosomal rearrangements (Myung et al., 2004). Thus, it is possible that functions outside of the spindle assembly checkpoint are compromised in BubR1 hypomorphic and Bub3/Rae1 haploinsufficient mice and that early onset of aging-related phenotypes is linked to such defects.
Although BubR1 hypomorphic mice age much faster than Bub3/Rae1 mutant mice, both models have a common spectrum of age-related phenotypes that is distinctive from that of mouse strains in which DNA damage repair or telomere maintenance defects cause premature aging (Hasty et al., 2003; Lombard et al., 2005). For example, mice with mitotic checkpoint gene defects develop cataracts at high incidence, whereas the other models do not. Conversely, mice with defects in DNA damage repair or telomere maintenance show hematopoietic stem cell depletion and glucose intolerance, whereas mice with a weakened mitotic checkpoint do not (unpublished data). This distinction supports the idea that the lesion that triggers aging in BubR1 hypomorphic mice and Bub3/Rae1 mutant mice is distinct from that of other aging models. This suggestion is further supported by the demonstration that DNA damage repair and telomere maintenance functions are seemingly intact in Bub3/Rae1 haploinsufficient mice and BubR1 hypomorphic mice (Baker et al., 2004).
In this study, we have used a modified nocodazole challenge assay to measure the duration of sustained spindle checkpoint activity of MEFs with various mitotic checkpoint gene defects. In essence, we treated MEFs with nocodazole and determined how long individual cells remained in mitosis by live cell imaging and then used this time as a measure for spindle checkpoint activity. We validated our nocodazole challenge assay by using a series of MEFs with a graded reduction of BubR1 protein level. We found that the duration of arrest in mitosis declined as the amount of BubR1 decreased. We found further that this time of arrest correlated well with the rate of aneuploidy and chromosome missegregation. However, the finding that Bub3/Rae1 double-insufficient MEFs are more aneuploid than BubR1H/H MEFs (the percentage of aneuploid cells is higher and the spectrum of chromosome losses and gains is wider) appears inconsistent, as we find that Bub3/Rae1 haploinsufficient MEFs sustain their mitotic arrest in nocodazole for twice as long as BubR1H/H MEFs. A possible explanation for this discrepancy is that the nocodazole challenge assay may not provide a reliable measure for spindle assembly checkpoint activity; the reasoning is that the checkpoint functions to protect cells against chromosome missegregation rather than against microtubule poisoning. Alternatively, increased aneuploidy in Bub3/Rae1 haploinsufficient cells might be caused by the dysfunction of proteins involved in chromosome segregation but not in the spindle assembly checkpoint. Examples of such proteins are AdAPC (adenomatous polyposis coli; Kaplan et al., 2001), securin (Jallepalli and Lengauer, 2001), and separase (Waizenegger et al., 2002; Chestukhin et al., 2003).
Abnormal numbers of chromosomes, termed aneuploidy, is one of the most common properties of cancer cells (Rajagopalan and Lengauer, 2004). Because aneuploidy occurs with such high frequency, it has been suggested that aneuploidy is an essential step in the process of tumorigenesis (Lengauer et al., 1998). In this study, we have demonstrated that Bub3+/–, Rae1+/–, and Bub3+/–/Rae1+/– mice do not show any differences in both the rate of tumor formation and the type of tumor formed in comparison with wild-type animals. An earlier cancer susceptibility study on Bub3+/– mice yielded similar data (Kalitsis et al., 2005). That study further showed that even on a p53 or Rb1 heterozygous background, Bub3 haploinsufficiency had no significant effect on tumorigenesis. A suggested explanation for these findings was that the degree of impairment of the checkpoint machinery was too low to cause substantial aneuploidy (Kalitsis et al., 2005). However, although the aneuploidy in our double heterozygous Bub3/Rae1 mice is quite severe at 5 mo, we find no increased susceptibility to lifetime spontaneous tumor development. This result is consistent with our earlier observation that BubR1 hypomorphic mice are not tumor prone despite having severe aneuploidy (Baker et al., 2004). So far, only Mad2 haploinsufficiency has been shown to increase the risk for spontaneous tumors in mice (Michel et al., 2001). However, several studies have shown that impaired spindle checkpoint function increases the risk for carcinogen-induced tumors. For instance, we have shown previously that Rae1+/– and Bub3+/–/Rae1+/– mice are susceptible to lung adenoma formation after early postnatal treatment with dimethylbenzanthrene (DMBA; Babu et al., 2003). Others have shown that BubR1+/– mice have a susceptibility to azoxymethane-induced intestinal and lung tumor development (Dai et al., 2004), and, consistent with this finding, we observed that DMBA-treated BubR1H/H mice are prone to lung tumors (Fig. S3, available at http://www.jcb.org/cgi/content/full/jcb.200507081/DC1). All of these studies illustrate an important point that challenging these mice with carcinogenic agents introduces cooperating mutations that synergize with mitotic checkpoint failure to cause tumorigenesis and that these mutations may not occur under normal situations. It seems that the identification of gene mutations that cooperate with spindle assembly checkpoint gene alterations in tumorigenesis will be important to provide a better understanding of the role of aneuploidy in cancer. The link between spindle assembly checkpoint impairment and cancer predisposition is further supported by a recent report showing that a high proportion of individuals with mosaic variegated aneuploidy (MVA) syndrome carry bi-allelic mutations in the BubR1 gene (Hanks et al., 2004). MVA is a rare recessive disorder that is characterized by severe aneuploidy and a high risk for neoplasms such as rhabdomyosarcoma, Wilms tumor, and leukemia (Kajii et al., 2001; Plaja et al., 2001). Other characteristics of MVA syndrome include microcephaly, growth retardation, and eye abnormalities such as cataracts. Whether MVA patients with bi-allelic mutations in BubR1 develop progeroid features other than growth retardation and cataracts remains to be established: so far, only four patients with BubR1 mutations have been identified, three of which are <3 yr of age.
In a recent study, Kalitsis et al. (2005) confirmed that MEFs from Bub3 haploinsufficient mice have increased aneuploidy, but the increase was not as profound as the one we previously observed (Babu et al., 2003). The smaller increase most likely results from the fact that chromosome counts were performed at P3 instead of P5. Furthermore, BubR1+/– mice generated by Wang et al. (2004) typically exhibited splenomegaly and extramedullary megakaryopoiesis, but we did not detect these phenotypes in our BubR1+/– and BubR1H/H mice (Baker et al., 2004). It is unclear why that is, but it might be the result of the different methods used to create the models (enhancer trapping vs. homologous recombination), differences in the mouse housing facilities, and/or possible differences in genetic background.
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Karyotype analysis
Metaphase spreads were prepared from splenocytes as described previously (Babu et al., 2003). The aneuploidy data for Bub3+/–, Rae1+/–, and Bub3+/–/Rae1+/– MEFs presented in Table II were first published in Babu et al. (2003); the data for BubR1H/H MEFs and mice were first published in Baker et al. (2004).
Collection and analysis of tumors
Moribund mice were killed, and major organs were screened for overt tumors using a dissection microscope. Collected tumors were processed for histopathology by standard methods. A chi square or Fisher's exact test was used to compare tumor incidence proportions across the genotypes for mice that developed tumors. Board-certified pathologists assisted in tumor analysis.
Cataract and body fat analysis
Biweekly, mice were screened for overt cataracts by examining dilated eyes with a slit light. At biopsy, cataractous eyes were collected and processed by standard methods for histological evaluation. Body fat content was measured using a small animal densitometer (Lunar PIXImus Corp.) as described previously (Nagy and Clair, 2000). A Mann-Whitney test was used for statistics.
Analysis of skin and skeletal muscle
Dorsal skin was dissected, fixed in 10% formalin, processed, and embedded in paraffin. 5-µm cross sections were prepared and stained with hematoxylin and eosin using standard procedures. Sections were stained, and the thickness of the dermal and adipose layers was calculated using a calibrated computer program (Spot Advanced; BioSpot). 40 random measurements were taken for each mouse of each genotype at the indicated age (n = 4 males). Histopathology on gastrocnemius muscle was performed as previously described (Kane et al., 2004; O'Cochlain et al., 2004). A Mann-Whitney test was used for statistics.
Generation and culture of MEFs
MEFs were generated from wild-type Bub3+/–, Rae1+/–, or Bub3+/–/Rae1+/– 13.5-d-old embryos as previously described (Babu et al., 2003). Growth curves of MEFs were generated as previously described (Baker et al., 2004). BubR1H/H, BubR1+/–, and BubR1+/H MEFs were previously generated (Baker et al., 2004).
Analysis of cell death, apoptosis, and DNA repair
TUNEL/Hoechst stainings were performed on P5 MEFs according to the manufacturer's protocol (Roche), as were annexin V/PI stainings (BD Biosciences). FACS analysis was performed as previously described (Tran et al., 2005). DNA damage survival experiments were performed as described previously (Baker et al., 2004).
SA ß-galactosidase staining
MEFs were stained with an SA ß-galactosidase kit according to the manufacturer's protocol (Cell Signal Technology). Nuclei were stained with Hoechst for visualization. The percentage of senescent cells is the total number of senescent cells divided by the total number of cells.
Western blot analysis
We performed Western blot analysis as previously described (Kasper et al., 1999). Antibodies for SA proteins were used at a 1:200 dilution (purchased from Santa Cruz Biotechnology, Inc. unless otherwise noted): p16 (M-156), p19 (NB200-106; Novus Biologicals), p21 (M-19), and p53 (FL-393-G). ß-actin was used as a loading control (1:50,000 dilution; Sigma-Aldrich).
Time-lapse live microscopy
To allow visualization of chromosomes by fluorescent microscopy on live cells, we used a retrovirus expressing YFP-tagged H2B that was constructed as previously described (Jeganathan et al., 2005). P2 MEFs cells were seeded in T25 flasks at 50% confluence and cultured in DME/10% FBS. The next day, these MEFs were grown for 24 h in medium harvested from EcoPACK cells that produce pMCSV-puro H2B-YFP virus. 24 h after transduction with pMCSV-H2B-YFP retrovirus, MEFs were seeded in 35-mm glass-bottomed culture dishes (MatTek Corporation). The next day, Hepes was added to the culture medium at a final concentration of 20 mM. 4 h later, the dish was placed in a heat-controlled stage of a microscope (Axiovert 200; Carl Zeiss MicroImaging, Inc.). The temperature was maintained at 37°C. CO2 levels were maintained at 10% using a controller (CTI 3700; Carl Zeiss MicroImaging, Inc.). For nocodazole challenge experiments, nocodazole was added to a final concentration of 100 ng/ml. During the next 30 min, 20–30 cells undergoing NEBD were marked by the use of a mark/find module (AV4 MOD; Carl Zeiss MicroImaging, Inc.). Subsequently, time-lapse sequences were captured by using a plan Apo 63x NA 1.4 differential interference contrast (D = 0.18) oil objective (Carl Zeiss MicroImaging, Inc.) and a camera (AxioCam Hrm; Carl Zeiss MicroImaging, Inc.). The exposure times in nocodazole challenge experiments were always 100 ms at 2 x 2 binning. Interframe intervals were 15 min except for wild-type, BubR1+/H, and BubR1+/– MEFs (intervals were 30 min). Imaging WS/20A software (Carl Zeiss MicroImaging, Inc.) was used to determine the time of arrest in mitosis of individual cells. The time of arrest in mitosis was defined as the interval between NEBD (onset of mitosis) and chromatin decondensation (exit from mitosis without cytokinesis). For analysis of mitotic defects, cells entering mitosis were marked, and images were acquired at 2- or 3-min interframe intervals until the completion of mitosis.
Indirect immunofluorescence and staining for poly(A)+ RNA
Indirect immunofluorescence for mAb414 was performed as described previously (Wu et al., 2001), as were stainings for poly(A)+ RNA (Bastos et al., 1996).
Online supplemental material
Fig. S1 shows that Mad2 haploinsufficiency does not accelerate cellular senescence or aging. Fig. S2 shows that Bub3+/–/Rae1+/– MEFs have no overt defects in nuclear pore complex architecture and mRNA export. Fig. S3 shows that BubR1H/H mice are highly susceptible to DMBA-induced tumor formation. Fig. S4 shows examples of BubR1H/H anaphases with lagging chromosomes. Video 1 shows a Bub3+/–/Rae1+/– cell undergoing normal mitosis. Videos 2 and 3 show mitotic Bub3+/–/Rae1+/– cells with one and two lagging chromosomes, respectively. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200507081/DC1.
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Acknowledgments |
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This work was supported by grants from the National Institutes of Health to J. van Deursen (CA77262 and CA96985).
Submitted: 18 July 2005
Accepted: 10 January 2006
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4 male mice per genotype). Bub3+/–/Rae1+/– mice have normal body weights at 5 mo but significantly reduced body weights at 24 mo. We note that neither Bub3+/– nor Rae1+/– mice show this reduction (not depicted). The decrease in body weight between the 24- and 35-mo-old wild-type mice is not significant but does show a trend of reduction in body weight. (B) Graph showing total body fat content in wild-type and Bub3+/–/Rae1+/– mice at 5, 27, and 35 mo (n = 4 male mice per genotype at each age). (C) Graph showing subcutaneous adipose layer thickness of wild-type and Bub3+/–/Rae1+/– mice at 5 and 27 mo along with 35-mo-old wild-type mice. Note the reduced thickness in 27-mo-old Bub3+/–/Rae1+/– mice and 35-mo-old wild-type mice (n = 4 males per genotype). (D) Graph showing dermal thickness of wild-type and Bub3+/–/Rae1+/– mice at 5 and 27 mo along with 35-mo-old wild-type mice. (A–D) Error bars represent SD. Asterisks mark values that are significantly different from wild-type values using a Mann-Whitney test. (E) Representative hematoxylin and eosin–stained dorsal skin sections of 5- and 27-mo-old wild-type and Bub3+/–/Rae1+/– from which the data in C and D were collected. Dermis (d) and adipose layer (a) are indicated. Bars, 100 µm.
-irradiation (C), UV type B (D), or paraquat (E). (F–H) Colony-forming assay of wild-type and Bub3+/–/Rae1+/– MEFs after DNA damage by 