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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200203043
Bunnell et al.
• Figure S1 Tyrosine phosphorylation during contact formation.
• Figure S2 The identification of signaling complex components.
• Figure S3 Cluster formation is favored in new areas of contact.
• Video 1 (1.4 MB) Rearrangement of the actin cytoskeleton during contact formation.
• Video 2 (1.5 MB) ZAP-70 is rapidly recruited into small contacts.
• Video 3 (2.8 MB) Small contacts are sufficient to initiate calcium influxes.
• Video 4 (3.1 MB) Small contacts are sufficient to initiate calcium influxes.
• Video 5 (644 K) Signaling adaptors are dynamically redistributed throughout contact formation.
• Video 6 (440 K) Signaling adaptors are dynamically redistributed throughout contact formation.
• Video 7 (440 K) Signaling adaptors are dynamically redistributed throughout contact formation.
• Video 8 (280 K) Signaling adaptors are dynamically redistributed throughout contact formation.
• Video 9 (780 K) Signaling adaptors are dynamically redistributed throughout contact formation.
• Video 10 (10.7 MB) Photobleaching reveals that ZAP-70 initially equilibrates between signaling complexes.
• Video 11 (9.9 MB) Photobleaching reveals that ZAP-70 initially equilibrates between signaling complexes.
• Video 12 (1.0 MB) LAT is selectively retained in signaling complexes.
• Video 13 (712 K) LAT is selectively retained in signaling complexes.
• Video 14 (1.2 MB) SLP-76 clustering is selectively induced by TCR ligation.
• Video 15 (1016 K) SLP-76 clustering is selectively induced by TCR ligation.
• Video 16 (888 K) SLP-76 clustering is selectively induced by TCR ligation.
• Video 17 (1.4 MB) The movement of SLP-76-containing clusters is microtubule dependent.
• Video 18 (644 K) The movement of SLP-76-containing clusters is microtubule dependent.
• Video 19 (4.0 MB) The SLP-76-containing clusters do not colocalize with transferring-containing vesicles.
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