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[HELP with Viewing Videos] [To Figure]
Video 3 A (6.2 MB) and
B (7.0 MB)
Corresponds to images
presented in Fig.
6. The looping and capture of microtubules contributes
to spindle morphogenesis in PTK
T
cells released from monastrol arrest. After
monastrol washout, the monopolar spindle transforms into a bipolar
spindle. As the centrosomes separate, the formation
and looping of microtubule bundles from the distal
end of an existing kinetochore is followed
by capture and incorporation into the spindle.
Frames were acquired every 30 s. Time
is shown in minutes:seconds. (A)
Microtubules are imaged by 3-D confocal fluorescence
microscopy and presented as a maximum intensity
projection of a 6-µm-thick Z-series in 1.5-µm
steps. (B) Chromosomes in the same cell are
imaged by 2-D DIC microscopy, and each image
is acquired immediately before (3 s) the fluorescence
Z-series at each time point.
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Video 3 A |
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Video 3 B |
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