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J. Cell Biol. 10.1083/jcb.200211015
Garvalov et al.

• Figure S1 Immunofluorescence analysis shows that in contrast to -actinin, VASP, or zyxin, endogenous Tes or GFP-Tes is found at focal adhesions and is not observed along actin stress fibers.

• Figure S2 Immunofluorescence analysis showing the localization of GFP-Tes-N-term, GFP-Tes-C-Term, GFP-LIM1, and GFP-Tes-C265A with respect to endogenous -actinin, VASP, or zyxin.

• Figure S3 Western blot of HeLa cells transfected with zyxin and control siRNA oligos with the indicated antibodies (left) reveals that the levels of endogenous Tes and Grb2 remain the same in the absence of zyxin.

• Video 1 (6.5 MB) Growth of wild-type T47D cells.

• Video 2 (7.3 MB) Growth of T47D cells stably expressing GFP.

• Video 3 (6.4 MB) Growth of T47D cells stably expressing GFP-Tes.

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