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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200304140
Peters et al.

Figure S1 PrPC was immunogold labeled on ultrathin cryosections of CHO/30C3 cells (steady-state distribution of PrPC) using the R1 recombinant antibody Fab fragment against PrPC.

Figure S2 Uptake of protein A-gold in SHaPrPC-expressing cells, after a 10-min pulse and 50-min chase before fixation.

Figure S3 The endogenous PrPC did not colocalize with the transferrin receptor.

Figure S4 Human GPI-anchored CD59 proteins in CHO cells under steady state do not reside in caveolae.

Figure S5 The bacterial toxin aerolysin, a monovalent probe for several GPI-anchored proteins, was used on ultrathin cryosections of PrPC-expressing CHO cells.

Figure S6 Actin distrubution near the plasma membrane caveolae of SHaPrPC-expressing CHO/30C3 cells.

Supplemental materials and methods (PDF)





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