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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200408165
Ang et al.
• Figure S1 Even without the 20°C TGN block, there is a intersection of VSV-G and Tfn in MDCKT cells after release of VSV-G from the 40°C ER block by incubation directly at 31°C for 20-30 min.
• Video 1 (456 K) 20°C rotating view and sagittal cross section of cells.
• Video 2 (312 K) 31°C rotating view and sagittal cross section of cells.
• Video 3 (316 K) Whole cell from which Fig. 2 (A and C) are based.
• Video 4 (2.3 MB) Close-up movie of entry of VSV-G (green)-containing structures (asterisk) into Tfn (red)-containing endosomes
• Video 5 (3.5 MB) Apical variant of VSV-G (green) also entering Tfn-positive structures prior to arrival at the cell surface.
• Video 6 (164 K) Close-up movie as VSV-G (green) exits the Tfn+ structures (yellow).
• Video 7 (2.3 MB) Whole cell followed by a close-up of the cell periphery showing that there is a persistent colocalization or tethering of VSV-G and Tfn.
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