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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200412081
Danen et al.
• Figure S1 Identical MTOC polarization in GEß1 and GEß3 cells 3 h after wounding.
• Figure S2 FLIP experiment on GEß1 and GEß3 cells stably expressing GFP-paxillin.
• Figure S3 FLIP analysis of GFP-paxillin in GEß1 and GEß3 cells transiently transfected with dsRed alone or in combination with HA-tagged cofilinS3A or cofilinS3D.
• Figure S4 Quantification of the induction of a morphological switch induced by transient expression of the indicated expression plasmids in combination with 10% GFP plasmid.
• Figure S5 Surface expression levels of ß1 and ß3 integrins as determined by FACS. c, control IgG; m, mouse; h, human.
• Video 1 (3.2 MB) Time-lapse CCD imaging of migrating GE11, GEß1, and GEß3 cells in wounding assay.
• Video 2 (2.3 MB) Time-lapse CCD imaging of randomly migrating GEß1 and GEß3 cells sparsely seeded on FN-coated coverslips.
• Video 3 (8.1 MB) Time-lapse confocal imaging of GFP-paxillin in GEß1 and GEß3 cells migrating into a wound.
• Video 4 (2.2 MB) Time-lapse confocal imaging of GFP-paxillin in GEß1 and GEß3 cells spreading on FN.
• Video 5 (1.0 MB) Time-lapse CCD imaging of randomly migrating GEß1 cells treated with Y27632 sparsely seeded on FN-coated coverslips.
• Video 6 (1.0 MB) Time-lapse CCD imaging of migrating GEß1 RacQ61L clone 1 cells sparsely seeded on FN-coated coverslips.
• Video 7 (1.3 MB) Time lapse CCD imaging of migrating GEß1 RacQ61L clone 1 and clone 2 cells transiently expressing GFP-tagged cofilinS3A sparsely seeded on FN-coated coverslips.
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