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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200412114
Wittmann et al.
• Figure S1 GSK3ß inhibition reduces the CLASP phosphorylation state in cells and induces ectopic MT lattice binding in EGFP-CLASP1-expressing cells.
• Video 1 (7.5 MB) 3xEGFP-CLASP2 in a PtK1 cell reveals MT plus end tracking in the cell body and dynamic lattice binding in the lamella.
• Video 2 (6.9 MB) 3xEGFP-CLASP2 (green) and X-rhodamine-labeled tubulin (red) in a PtK1 cell demonstrating that all lamella MTs are CLASP decorated.
• Video 3 (7.9 MB) Comparison of the dynamic behavior of EGFP-tagged CLASP2, EB1, CLIP-170, and APC in PtK1 cells.
• Video 4 (1.3 MB) FRAP of EGFP-CLASP2 on lamella MT lattices (left) and growing plus ends in the cell body.
• Video 5 (8.4 MB) 3xEGFP-CLASP2 in a PtK1 cell expressing constitutively active mRFP-Rac1(Q61L) showing randomized plus end tracking and lattice binding in both cell body and lamella.
• Video 6 (5.2 MB) 3xEGFP-CLASP2 in a PtK1 cell treated with a Rac1 inhibitory peptide, TAT-Rac1(17-32), which inhibits MT lattice binding.
• Video 7 (4.5 MB) 3xEGFP-CLASP2 in a PtK1 cell expressing constitutively active mRFP-GSK3ß(S9A).
• Video 8 (4.7 MB) 3xEGFP-CLASP2 in a PtK1 cell in the presence of 20 mM lithium chloride, which induces ectopic MT lattice binding in the cell body.
• Video 9 (4.7 MB) Truncated 3xEGFP-CLASP2(78-875) only tracks growing plus ends in PtK1 cells.
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