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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200503028
Wu et al.
• Figure S1 Estimation of Mlp over expression and the interaction of Mlp and Mlp fragments with EB1 in vitro and in vivo.
• Figure S2 Correlation between Mlp plus end tracking behavior and overexpression and the plus end tracking behavior of Mlp mutants and various spliced isoforms of myosin Va.
• Figure S3 Model for how the plus end complex of EB1-Mlp-myosin Va might serve to focus the transfer of melanosomes from microtubules to actin at the microtubule plus end.
• Video 1 The dynamic behavior of Mlp-GFP in a primary mouse fibroblast.
• Video 2 Mlp-mRFP tracks the growing end of microtubules that were visualized with GFP-tubulin (primary fibroblast).
• Video 3 EB1-GFP and Mlp-mRFP track the plus end together (primary fibroblast).
• Video 4 A low dose of nocodazole blocks Mlp-GFP plus end tracking (primary fibroblast).
• Video 5 Mlp-GFP in a primary wild-type mouse melanocyte where the protein has targeted to both melanosome and the microtubule plus end.
• Video 6 Mlp-GFP plus end tracking behavior in a lightly transfected melan-c melanocyte that was used for correlative video/quantitative immunofluorescence.
• Video 7 Mlp-GFP in primary wild-type melanocytes where the protein has targeted almost exclusively to either the plus end/actin or to melanosomes
• Video 8 Mlp-mRFP and MCMVa-GFP track the plus end together (CV1 cell).
• Video 9 The dynamics of Mlp(-)RBD-GFP in melan-c melanocytes
• Video 10 Mlp491-590-GFP tracks the plus end, whereas Mlp1-490-GFP does not (CV1 cell).
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